FastQCFastQC Report
Sat 2 Sept 2023
SRR12318326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12318326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46252019
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT21637694.678215236398653No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG14828383.2059962614821207TruSeq Adapter, Index 4 (100% over 49bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC12488372.700070239095941No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC11907452.5744713976702296No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT4663571.0082954432756763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG4301510.9300156172641891Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA3970680.8584879289269512Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG3141270.679163865257428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG2849310.6160401343777014Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA2833530.612628391422221Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC2831240.6121332779007982No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2719690.5880154118245087Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2426940.5247208775902301Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT2373020.5130630081251155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC2185340.4724853200462449No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2104740.4550590537463889No Hit
GCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAG2074540.44852960905339073Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1944170.4203427314167626No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT1942360.4199513971487385No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGA1902420.4113160984388595Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC1653150.357422234908275No Hit
AGTCCGCCCGGAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1623750.35106575563760795Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1614270.34901611538298466Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1501570.3246496115120942Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1388480.3001987869978173No Hit
GGGGAGGAAGGGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1370580.2963286856731595Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1326680.28683720812274166No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG1325520.2865864082603615No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1288760.27863864710424857Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1251320.27054386533915414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1241630.268448821661169Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1199970.2594416472932781Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1158730.25052527977211114No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA1154790.24967342506713058Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1137140.24585737543695121Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1090060.23567836033276732Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT999960.21619812964272975No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC946550.2046505256343512No Hit
CGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAACT855620.18499084331864518Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT834380.18039861135575508Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGT792070.17125090258222025Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAAC781530.1689720831430083Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTCT758470.163986354844315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT753850.16298747953035303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA746260.16134647008598696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGA709510.1534008709976531Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA694610.15017939000673677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT676730.14631361281763722Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC656860.1420175841404891No Hit
CCGCCGAGGGCGCACCACCGGCCCGTAGATCGGAAGAGCACACGTCTGAA584890.12645718233402956Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA577650.12489184526193332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC572460.12376973208456046No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG567360.12266707751719984No Hit
CGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTGAA555710.12014826855450353Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTGA493280.10665047940934211Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA471950.10203878883643977No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCT470630.10175339588959348Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTG463540.10022048983418433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT462970.10009725197077342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)