FastQCFastQC Report
Sat 2 Sept 2023
SRR12318328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12318328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28346186
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC7487182.641335945513093No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6841432.41352752006919No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4422481.5601675654001566No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3904081.377285818981079No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG3322201.1720095253731844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA3270371.153724878542743Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2927741.032851474268884No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA2716130.9581994558280257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG2518590.8885110681204166Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT2109050.744033077324759No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC1962100.6921918878257555No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1533840.5411098339649645No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1459520.5148911391465504No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1422240.5017394579997464No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG1405380.4957915678673667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1365780.48182143446035386Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1347510.47537612291120923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC1339090.4724057056564858No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT1281510.45209256723285457No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1115650.3935802862508557No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG1036930.3658093543872181No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG1024290.3613502006936665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT989090.3489323043318773No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA983720.3470378695744112Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA939750.33152608255657395Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA936650.33043246100198453No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT923360.32574399956311584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA789490.2785171874621863No Hit
GGATTCAACCCGGCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT788940.27832315783153333Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT779260.27490823633204126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT707530.24960324468342934No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGA691560.2439693297715608Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG657840.2320735495068014No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC656840.23172076836015962Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC653260.23045781185518222No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC586610.20694494843151034No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT538230.18987739655698302Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC532680.18791946119312136No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT531700.18757373566941243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC511680.18051105711364485No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT492480.17373765909812344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG476320.16803671576839296Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC468520.1652850228245874No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA464210.1637645360825615No Hit
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC458840.16187010132509536Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCA454750.16042722643533067Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG449410.1585433751122638No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT446210.1574144754430102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC428140.151039720123194No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG427200.15070810584535077No Hit
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA427060.15065871648482093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATTCAACCCGGCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC413600.14591028225102312Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT396100.1397366121847927Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTCT377190.13306552070179742Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCTG363800.12834178114826456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG362820.12799605562455563No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTAGATCGGAAGAGCACACGTCT351300.12393201681524278Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT345580.12191410865645205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT343130.12104979484717979No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA337910.1192082772617099Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACAC330620.11663650270269164No Hit
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAAC325520.11483731885481878Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAAC319200.112607742008043Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCCAGATCGGAAGAGCACACGTCTGAA316030.11148942577318868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCT310610.10957735195839045Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC308380.10879065000137936No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG308100.1086918712803197No Hit
GATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG306740.10821208892088693Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCTG305840.10789458588890935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC299930.10580964931225667Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC295140.10411982761984276No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTGA294250.10380585239933161Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)