FastQCFastQC Report
Sat 2 Sept 2023
SRR12318329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12318329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35123808
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT14573124.149071763517213No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA5679971.6171281883786632No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5500931.5661542165359748No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG4382481.2477234814630578No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3098170.8820712150573196No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG3020330.8599096088897877Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA2816760.8019517701497515No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2444080.6958471017721085No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT2307740.6570301261184437No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2091480.5954593533821846No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2084540.5934834856175049No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC2008170.5717403989909067No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1766260.5028668873261122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1605820.4571884688585019No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1526330.43455709585930996No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1389660.39564616683931314No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT1341590.38196029314361357Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1242900.35386254246692156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT1090780.3105528876595613No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1018920.29009383037283426No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC980960.2792863461729434No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCAC976750.27808772898428324No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT969910.2761403319366738Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACG963730.2743808416217285No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACGT939090.2673656569356033No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG929900.26474919803684155No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG892800.25418656200375545No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCA892650.2541438559281499No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG879290.2503401681275561No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT845060.24059464167438793No Hit
GTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACACG794820.2262909534182626No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC792660.22567598592954388No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC780670.22226234695281333No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC764530.21766717321766477No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG744570.21198441809043028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC714150.20332362595764103No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC695000.1978714836386761No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT689060.19618032304469946Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC670710.19095594646229702No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG641650.18268235608166403No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC621140.17684301201054284No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG596790.16991039240392158No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT570670.16247384110515578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC547440.15586009352972205No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC546300.1555355273551205No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT508020.14463693686060464No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT496230.14128023931801473No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG493360.14046312973809674No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA483800.1377413291861748No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA471570.1342593604884755No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG459920.13094252194978404TruSeq Adapter, Index 9 (100% over 49bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTC447310.12735236452721754No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC417610.11889656155733455No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT417070.11874281968515486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG410830.11696624693996734Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC397130.11306575870133444No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC395720.11266432159064302No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA394350.11227427276677973No Hit
GCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACAC382040.10876952749542419No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGAGATCGGAAGAGCACACGTCT380150.10823143094279526Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGATCGGAAGAGCACACGTCT379210.10796380620233433Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC369810.10528755879772489No Hit
ATCATTGATGCCCCAGGACACAGAGACTTAGATCGGAAGAGCACACGTCT369320.10514805228408036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG360440.10261985260823654Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC359940.10247749902288499No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCACACG358850.10216716820681858No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG356130.10139276470250606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)