FastQCFastQC Report
Sat 2 Sept 2023
SRR12318330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12318330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32225196
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT9445342.931041908946031No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC8755362.7169299451274087No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC5297171.6437976048307046No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG4397751.3646930184691506No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4238401.3152441338138021No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA4066971.2620466296000186No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2955440.9171208764719383No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2895350.8984739766982333No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2669090.8282618358628446No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT2414580.7492832626991625No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG2352780.7301057222429307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC2344710.7276014706008305No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT2251830.6987793030025325No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA2077510.6446849850036599Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1902850.590485159500659No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1774070.5505226407311844Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1761020.5464730144697956Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA1598820.49613972867690237No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1539150.4776231617024145No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG1521440.47212746200209305No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC1372960.42605171431695865No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT1313730.40767168646546015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC1288640.3998858532931809No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC1274460.39548556973866045No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC1245060.3863622737934627No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT1139350.3535587494952707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC1097470.3405627075161932No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT973830.3021952139561851No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA956500.2968174344075363Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG953580.29591131113678876No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG836760.2596601739831156No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACGT831530.258037220316674No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG814930.252885971585712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC801450.24870290936322No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT797910.24760439005553295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA756190.2346579986666334No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACG743980.23086903800367883No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA728430.2260436212707597No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG720200.22348971903848155No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC714000.22156575866908615No Hit
GGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACTC700310.2173175300469856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC697340.21639589096680745No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC689410.21393508359111296No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT642750.19945573023046936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC611850.18986696000235342No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC594300.18442091089220994Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG589060.18279485406388218No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCAC570200.17694229074665674No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG556790.17278095065736762Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT555360.17233719850765222No Hit
TGGCGGTGCGCCGCGACCGGCTCCAGATCGGAAGAGCACACGTCTGAACT539420.1673907584611743Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG538770.1671890529385764No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT526180.16328217212394922No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG519340.161159609393842TruSeq Adapter, Index 10 (100% over 49bp)
TCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGAGATCGGAAGAGCACAC516760.16035899362722264No Hit
GTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACACG512840.15914255416786294No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT500870.1554280693901753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC480990.14925898356056547No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCGAGATCGGAAGAGCA474550.14726054730590313No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT467320.14501696126223718No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC464480.14413566328657862No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC448030.1390309619839085No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT442900.13743903993632808Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGTGCGCCGCGACCGGCTCCGAGATCGGAAGAGCACACGTCTGAACT430180.13349181801718135Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC423980.13156785764778592No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC411200.12760201675732244No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC410950.12752443771016939No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA374100.1160892861598111Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA369860.11477354552009553No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC365320.11336471002379629No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC336720.10448966702948836Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTCT334400.10376973347190814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG330610.10259363511706802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG327990.10178060670290413No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC324520.10070380952841994Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)