FastQCFastQC Report
Sat 2 Sept 2023
SRR12318332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12318332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47326420
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG22392734.731549523500827No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC16633903.514717572129901No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT11552912.4411121737076247No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT9432391.9930495482227477No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC5226171.1042817098779074No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC5154241.0890830111383873No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA4996311.0557126442270512Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT4640480.9805263106738266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4097360.8657658872147946Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC4059080.8576773818936654Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG3057260.6459943515693771No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2613640.5522581255882021No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2545980.5379616713032593TruSeq Adapter, Index 7 (100% over 49bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT2413100.5098843309931325No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2378900.5026579234178288No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2121210.44820842142718587Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2020450.4269179878807651Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1987070.4198648450484951Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1812510.38298058462905077No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG1644370.34745286036847917No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1545760.3266167185263538No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA1543120.32605889057317244No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1431090.3023871233023753Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC1426090.30133063096680457No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1311060.277024968294665No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1287930.27213763475031494No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1201700.2539173679310626No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1068450.22576184718810338No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1039890.21972716296732353No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1039650.21967645133521616No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT1035090.21871293032517564No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT1021190.21577588163228914No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1017840.21506803176745676Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC997460.21076176900767055No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG956290.20206261111658136Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC918410.19405862518229777Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT914910.19331908054739827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG899210.19000169461370625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA865060.18278585196175837Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGAGATCGGAAGAGCACACGTCTG809590.17106512599093698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT808080.17074606530559464No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC797020.16840910425931224Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCAGATCGGAAGAGCACACGTCTGA785570.16598973681085535Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA722060.15257017116443627No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG710190.15006205835979142No Hit
GACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTG697360.147351099026717Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA670700.14171788189345402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC668640.14128260705119888No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC652670.13790817053138607No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT651440.1376482734168357No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT640020.13523524492239217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGAGATCGGAAGAGCACACGTCT616530.13027184392988103Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTC607780.1284229823426323No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG590500.12477174483089995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT589520.1245646723331281Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCT551190.11646560208864308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC540130.1141286410423607No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTC538950.11387930885116601No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG514270.10866446268278902Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)