FastQCFastQC Report
Sat 2 Sept 2023
SRR12318333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12318333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26174044
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG6637542.535924521254721No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4988621.9059416267505318No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT4014911.5339280395494101No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT3364681.2855025383162035No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG3334591.274006416433013No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3330901.2725966228222128Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG2523380.964077236211569No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2375570.9076052596228538No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1817950.6945621394997272Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1788650.68336784334893No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1643470.6279006790085628No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1536280.5869478938753216Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1280980.48940851478663366Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1265290.48341402650656506No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1103070.42143659573583664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG1088400.41583180650265583No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC991620.3788562439950051No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC902060.34463913944669766No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC890400.34018434445972506No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA843410.3222314442506477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC833380.3183994036229174No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC814050.311014224626504No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCAGATCGGAAGAGCACACGTC804230.30726241615548594No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG792740.30287257100966136Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC788080.3010921812464287Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC745040.2846484096993189No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG691600.26423123610550975No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA684640.2615721131973339Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA683110.26098756462700223Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG657510.251206882665896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT643930.24601853653184047No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA615740.23524832463795048No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT610410.23321195608901704No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGAGATCGGAAGAGCACACGTCT580880.22192978662372542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT551500.2107049258417996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA543740.20774015662233927No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG532190.20332738800316832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT511130.19528124885860204No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG496950.18986366799108306No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT492820.18828576890907647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC486950.18604308910002595Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC482310.18427034049457547No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT471450.18012119181888744No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT457580.17482204889699124No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC446250.17049333301342354No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT424600.1622217797142849Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC424590.16221795913539383No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT421810.16115583820367996No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT410570.15686150753013176No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC403570.15418710230639177No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC396450.15146685013595912No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT382830.14626322168633932Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA373250.14260310710870663Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT350820.13403354865606554No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG350600.13394949592046226No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT349420.1334986676113175No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG333360.12736281791227982Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA333270.1273284327022603Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA326930.12490618568533009No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT311880.11915621445428914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC308740.11795655268249722No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT307050.11731087484990856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC306290.11702051085418821No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTG293790.11224478724036684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT289020.11042237110933258No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG282660.10799248293462027No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT273910.10464947640494529No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA262370.10024052836466539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)