FastQCFastQC Report
Sat 2 Sept 2023
SRR12318334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12318334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25999952
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG6823382.624381768089418No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT6077452.3374850845878483No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT4871801.8737726900418892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC4688101.8031187134499325No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC3904901.5018873880997934No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3733511.4359680356332967No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2601741.0006710781619905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2157530.8298207627460235No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1915230.736628283006061Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1833860.7053320713822856No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1799170.6919897390579798Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1738610.6686973883644093No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1630830.6272434656802444No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1581400.6082318921204162No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1515260.5827933836185544Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1503840.578401067817356No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT1417080.5450317754432777Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCAGATCGGAAGAGCACACGTCTGA1409810.5422356164349842Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTC1327320.5105086347851718No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1273650.48986628898391815Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCTG1194780.4595316175968324Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1185490.45595853407729364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC1122950.431904643516265No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCAGATCGGAAGAGCACACGTC1061990.4084584463848241No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG936920.36035451142371344Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGAGATCGGAAGAGCACACGTCTG885340.3405160132603322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC862150.3315967660247988No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC797180.3066082583537077No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC793030.3050121015608029No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG783050.3011736329359377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC729430.2805505179394177No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT726240.27932359259740175No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC661010.25423508474169493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGAGCCCGGCGGGTGCCGGCAGATCGGAAGAGCACACGTCTGAAC656490.25249661999376Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC645120.2481235349972954Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC639610.24600430031563136No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT620150.238519671113239No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGAGATCGGAAGAGCACACGTCT602070.2315658121214993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA587320.22589272472503028No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC577230.22201194832975074Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA497730.19143496880301933No Hit
GGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCA478770.1841426476479649Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCTG474110.18235033664677536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG462720.1779695593284172Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACG461250.1774041736692437No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCAGATCGGAAGAGCACACG457640.17601570956746382No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT444020.1707772383579785No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG412500.15865413905379516Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT409930.1576656756904782Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG399680.15372336072005055No Hit
CCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTCT393400.15130797164548612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG390260.1501002771082039Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG386260.14856181272950042TruSeq Adapter, Index 11 (100% over 49bp)
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG376920.14496949840522783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG376820.14493103679576025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC370130.14235795512237867No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA369420.14208487769515882Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC354920.13650794432235874No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCT350820.1349310183341877Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATTCAACCCGGCGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT341140.13120793453772528Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC334760.12875408385369325No Hit
GACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGTCTG323730.12451176832941845Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT303810.11685021572347518No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA299050.11501944311281805No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAACT291030.1119348220335176Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA289260.11125405154594131No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTC285740.10990020289268226No Hit
GATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG280580.10791558384415478Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT277510.10673481243349987No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG271560.10444634667017846No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG269690.1037271145731346No Hit
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC269400.10361557590567859Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA263280.10126172540626228Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTCCAG262170.10083480154117208Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)