FastQCFastQC Report
Sat 2 Sept 2023
SRR12318335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12318335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32436916
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT12476113.846268862304912No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8125692.505074773446403No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT4822021.4865839896739876No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4530671.3967634900925845No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3848691.1865153888242643No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT3335611.0283375891838793No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG3059010.9430643776368877No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA2886800.889973633744959No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2801250.8635993631453743No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2417190.7451972314507335No Hit
CGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC1998290.6160542512734565No Hit
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1986830.6125212396887546Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1769640.5455635794722284No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG1631380.5029393053273005Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC1563940.4821481795618301No Hit
TTGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC1494430.4607188920179711No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT1480620.4564613972549055No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1448350.4465128559077564No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT1419100.4374953525174835No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT1399960.43159466824774584No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG1320020.40694990855480834No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG1281100.39495123395824683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT1278780.39423599950130894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1247910.38471906515403625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1242150.3829433106402594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA1238600.3818488786048587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1174210.3619980395176903Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1070650.330071453155411No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG1025280.3160843034522764No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA983000.30304977205601175No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC956160.29477524928695437No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT891180.2747425186784095No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG884420.2726584734504353No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT832700.2567136777121475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG815760.2514912330136441No Hit
GTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG798970.24631503192227028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTC784230.2417708267950011No Hit
CAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG776700.23944939771709495Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC758320.23378301439014731No Hit
TGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG758240.23375835113301152Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG756160.233117106447481Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT713330.21991301515840778No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT708590.2184517171731123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA704600.2172216372234648Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC700420.21593298203811975No Hit
AGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG700220.2158713238952803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT699860.21576033923816923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC677410.2088392127044384No Hit
TGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC666310.20541718577684762No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC642530.1980860325932342No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG642070.19794421886470343No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAGATCGGAAGAGCACACGTC622500.19191096958786094No Hit
CTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG594290.18321408854035323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT563070.1735892524431114No Hit
GAGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC555470.17124624301521144No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG552020.17018264005123052No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT551020.16987434933703316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTG544090.16773789468764538Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC542690.1673062876877691No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC527700.16268500988195056No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA522730.16115280503238963Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG522420.16105723491098844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG515020.15877588362592793No Hit
TTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG506730.15622015360523175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA505480.15583479021248506No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG498870.15379698859164045Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC494860.152560742827709No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCAGATCGGAAGAGCACACGTC494200.15235727095633875No Hit
TCGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG493260.15206747768499324Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA483320.1490030679858714Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT478830.14761884267912523Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAA475870.14670630216510103Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA457130.14092893418104238No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT444740.13710921223213698Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC439910.1356201680825637Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC438080.13505599607558252No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT437620.13491418234705174Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG432610.13336964586892291Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG430890.13283938584050345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC430740.13279314223337385No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG416600.12843391153462308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT399180.12306348729330494No Hit
CTGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC393740.12138638580807128No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTAGATCGGAAGAGCACACGTCTG390780.12047384529404706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC380220.11721829535212287No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA374800.11554735968117315No Hit
ACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAA365020.11253227649632289Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGTACACGGAGTCAAGACCCGCAACGCGAAGATCGGAAGAGCACACGTC353240.10890061188307792No Hit
ATGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG349940.1078832525262266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTAGATCGGAAGAGCACACGTCTG342420.10556490635546241Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC337080.10391863394164846No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC335900.10355485089889556No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC334750.1032003165775686No Hit
TTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGTC330540.10190241267079769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)