FastQCFastQC Report
Sat 2 Sept 2023
SRR12343630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86416720
Total Bases10 Gbp
Sequences flagged as poor quality0
Sequence length72-122
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT12525531.4494336281219653No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA8690631.0056653388372065No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA6619490.76599644142939No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAAT4935130.5710850863120007No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC4295850.4971086613794183No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA3945650.45658409622582297No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC3848650.4453594165573514No Hit
ATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGATCGGA3848220.4453096576680994No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG3574800.4136699472046613No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG3385090.3917170195767671No Hit
AGCTCTCTGGGTCCCTACCGACGCTGCTGTAGGCACCATCAATAGATCGG3346220.3872190474250816No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATA3331010.3854589713657265No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAGATCG2888950.3343045188477415No Hit
AAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCAA2883240.33364376708581395No Hit
TGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC2631280.3044873723510913No Hit
ACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAATAG2375460.2748843047965718No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA2297870.2659057182452655No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTCTGTAGGCACCATCAATAGA2293450.26539424315109394No Hit
TGAGATTCAGCCCTTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAG2270940.2627894231579259No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA2143120.24799830403190493No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG2031890.2351269522842339No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGA1970590.22803341760714826No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG1873270.21677170806760543No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA1814880.21001491378057394No Hit
TTGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCA1770940.20493024960910344No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG1725790.19970556623764477No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCATCAATAGAT1699100.1966170435536086No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG1698360.19653141197675636No Hit
TAGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAA1640530.18983941996409953No Hit
TTTTGATCCTTCGATGTCGGCTCTTCCTACTGTAGGCACCATCAATAGAT1597920.18490866119426888No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTACTGTAGGCACCATCAATAGATC1590860.1840916896637595No Hit
TGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT1534920.17761840532711726No Hit
TTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCCTGTAGGCACCATC1389520.1607929576591197No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCAT1373400.15892757790390563No Hit
GCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAG1350650.1562949855074342No Hit
ATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGAT1300980.15054725520709417No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA1253680.14507377738937557No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACTGTAGGCACCATCAAT1249280.14456461666214593No Hit
AAAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCA1191600.13788998240155378No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT1163030.13458390922497404No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTATCTGTAGGCACCATCAATAGAT1152900.1334116823688749No Hit
TAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCA1138890.1317904683260369No Hit
TTGAGATTCAGCCCTTTGTCGCTAAGATTCGACTGTAGGCACCATCAATA1117160.12927590864360508No Hit
CCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC1089970.12612952678602013No Hit
ACCGGGACGTGGCGGTTGACGGCAACGTTCTGTAGGCACCATCAATAGAT1077080.12463791729193147No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG1071920.12404081062090762No Hit
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAATA1052880.12183753329216847No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG1016630.11764274320987883No Hit
TTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAAT1011000.11699124891571908No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTTCTGTAGGCACCATCAATAG1009450.11681188547771773No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA1005380.11634091180503033No Hit
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA989470.1144998329027068No Hit
AAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCA989370.11448826106799702No Hit
AGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT978150.11318990121356146No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA976010.11294226395077249No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA973620.11266569710120912No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGCTGTAGGCACCATCAATA957310.11077833086004653No Hit
GCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATC953880.1103814169295016No Hit
TAAGATTAGCATGGCCCCTGCGCAAGGATGCTGTAGGCACCATCAATAGA922830.10678836225211973No Hit
TCAGCTCTCTGGGTCCCTACCGACGCTGCTGTAGGCACCATCAATAGATC920790.10655229682404053No Hit
ACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATCAATAG903970.10460591422585815No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAGA902340.10441729332008898No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG894270.103483446259011No Hit
TGCATTTATTAGACTGATGATTCATGATAACTCCTGTAGGCACCATCAAT891800.1031976219416798No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA889850.1029719711648394No Hit
TTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATA876320.10140630192860826No Hit
TCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATC873700.10110311985921241No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT865470.1001507578625988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)