FastQCFastQC Report
Sat 2 Sept 2023
SRR12343633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4538898
Total Bases326.8 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCCGTGTCGGCGGTTCGATTCCGTCCCTGTAGGCACCATCAATAGAT927072.042500183965359No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC486581.0720223278866368No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA459911.0132635719066612No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT325270.7166276924486956No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA288080.6346915044136264No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA253590.5587038968489708No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT249100.548811627844468No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG247610.5455288926959804No Hit
TATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAG242630.534557066494995No Hit
AAAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCA242170.5335436046370727No Hit
AAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAGATCGGAAG166500.3668291290088475No Hit
TTGAGCTAACCGATACTAATTGCCCGTGCTGTAGGCACCATCAATAGATC139830.30807037302887175No Hit
TAGGACGGAGCACGAGAAACTTTGTCTGACTGTAGGCACCATCAATAGAT135690.2989492163075707No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG134820.2970324514893262No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA132610.29216342821539504No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA127220.2802882990540876No Hit
ATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGAA126880.27953921855040587No Hit
ACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA124220.27367876519807227No Hit
ATCCGTGTGTCGACAGTTCGATTCTGTCCTGTAGGCACCATCAATAGATC123920.27301781181247076No Hit
AGGTCCTAGCGGTGGCAACACCGTGGAAGTTCGAGTCTTCTCCTGTAGGC123690.2725110808835096No Hit
AAGAAGCGCATACGGTGGATGCCTTGGCCTGTAGGCACCATCAATAGATC122370.2696028859868629No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA118430.2609223648559628No Hit
ATCCCCGTGTCGGCGGTTCGATTCCGTCCCTAGGCACCATCAATAGATCG103250.22747812354452557No Hit
AGTGAAATTGAAATCGCTGTGAAGATGCCTGTAGGCACCATCAATAGATC96860.21339981643121306No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG93960.20701060037039828No Hit
ACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGG92940.20476335885935307No Hit
TTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAAT92180.20308894361582924No Hit
ATTGCGATGGTCCCTGCGGATGCTCTGTAGGCACCATCAATAGATCGGAA88930.19592861527181266No Hit
TAGGACGGAGCACGAGAAACTTTGTCTGCTGTAGGCACCATCAATAGATC85550.18848187379403547No Hit
ATCCTTGTGTCCCTGGTTCGATTCCTGTAGGCACCATCAATAGATCGGAA76420.168366859092229No Hit
TAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAGATCGGAAG74390.16389440784965867No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC73460.16184545235429393No Hit
ATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAGATCGGA69880.15395807528611571No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC65390.14406580628161283No Hit
TTATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATA62620.13796300335455874No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA60490.13327023431678792No Hit
TTGGTTGATGCCATGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGATC60470.1332261707577478No Hit
TCGGGGCTCAAACCACACACCGAAGCTACGGGCTGTAGGCACCATCAATA58740.12941467290077902No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTTCTGTAGGCACCATCAATAG58060.12791651189341555No Hit
TTATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGA57980.12774025765725514No Hit
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAG57390.12644038266557212No Hit
TTGGTTGATGCCATGCTTCCAGGAAAAGCTTCTAAGCTCTGTAGGCACCA56800.12514050767388912No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA53530.11793611577083248Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA53400.11764970263707182No Hit
ATCCCCGTGCCGGCGGTTCGATTCCGTCCCTGTAGGCACCATCAATAGAT53320.1174734484009114No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAGA52880.11650405010202916No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAG52030.11463134884282485No Hit
TAAGAAGCGCATACGGTGGATGCCTTGGCCTGTAGGCACCATCAATAGAT51490.1134416327487421No Hit
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA51270.11295693359930098No Hit
TTGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCA51250.11291287004026088No Hit
AGCGGTGGCAACACCGTGGAAGTTCGAGTCTTCTCCTGTAGGCACCATCA49360.10874886371097126No Hit
TTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC49050.10806587854584968No Hit
ATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGATCGGAAG45970.10128009045367399No Hit
TTCGAGTAGGACGGAGCACGAGAAACTTTGTCTGAACCTGTAGGCACCAT45890.10110383621751359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)