FastQCFastQC Report
Sat 2 Sept 2023
SRR12343635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11080571
Total Bases797.8 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC1677711.5141006722487498No Hit
TCGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG1522461.3739905642046786No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG1328031.1985212675411763No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATCAATAG1297121.1706255932117577No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCAT1201621.084438699052603No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA1140181.0289902930092683No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA1081510.9760417581368326No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAAT963240.8693053814645474No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT851320.7682997563934205No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA820550.7405304293433975No Hit
TCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATC766280.6915528089662527No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTACTGTAGGCACCATCAATA678260.6121164694490925No Hit
TAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATCAATA626890.5657560427165712No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACC601460.542805961894924No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA538000.48553454510602384No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC535830.4835761622753918No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG531460.4796323221971142No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT432940.39071993672528244No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG432180.39003405149427767No Hit
GCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATC423750.3824261403135272No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACTGTAGGCACCATCAAT354420.3198571625956821No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAAT347260.3133954017351633No Hit
AAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCAC335060.30238513881640217No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA334840.3021865930916376No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA333600.3010675171884193No Hit
TGTTCGGATTGTTCTCTGCAACTCGGGAACATGAAGTCTGTAGGCACCAT333270.30076969860127245No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA323180.2916636696791167No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA314680.28399258485866835No Hit
TAGGGCACCACCAGGAGTGGAGCCTGCGGCTTACTGTAGGCACCATCAAT300490.2711863856113552No Hit
AGCTCTCTGGGTCCCTACCGACGCTGCTGTAGGCACCATCAATAGATCGG289590.2613493474298392No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG286750.2587863026192423No Hit
TAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACC285330.25750478021394385No Hit
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT281400.2539580315851954No Hit
GTTCGGATTGTTCTCTGCAACTCGGGAACATGAAGTCTGTAGGCACCATC276350.24940050472128197No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGCTGTAGGCACCATCAATA276130.24920195899651743No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTCTGTAGGCACCATC270500.2441209934036793No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC259360.23406735988605643No Hit
AGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCA245490.22154995442021896No Hit
TTGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCA243580.21982621653703588No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCAA239560.21619824465724738No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAACTGTAGGCACCATCAAT231440.20887010245230145No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA226910.204781865483286No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT225450.20346424385530312No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCATC220530.19902403946511418No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAGA218740.1974085992499845No Hit
TTGGCGCTTAAGCGCGCGACCTATACCCGGCCTGTAGGCACCATCAATAG213560.19273374991234657No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA212080.19139807867302147No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT186900.16867361799315217No Hit
GAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATCG185790.16767186456365832No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC183980.16603837473718636No Hit
TAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGAT183170.165307365477826No Hit
GGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATCAATAG181510.1638092477364208No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATA179850.1623111299950156No Hit
TTGGCGCTTAAGCGCGCGACCTATACCCGGCTGTAGGCACCATCAATAGA172250.1554522776849677No Hit
TTCGGATTGTTCTCTGCAACTCGGGAACATGAAGTCTGTAGGCACCATCA160030.1444239651548643No Hit
GCCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAA158650.14317854197225033No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCAATAGA156310.14106673744520928No Hit
CGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC151530.136752880334416No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT150300.13564282923686874No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCA150120.13548038273478868No Hit
AAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATCGG146280.13201485735708024No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCAT145720.13150946823949777No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAAT145290.13112140159563979No Hit
AAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCAA144630.13052576442134617No Hit
CGAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATCA140240.12656387473172637No Hit
AAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACC139870.1262299569218951No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG137730.12429864850827634No Hit
CGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAGA135640.12241246412301315No Hit
ATGGCGCTTAAGCGCGCGACCTATACCCGGCTGTAGGCACCATCAATAGA134420.12131143783113704No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAAT134320.12122118977442589No Hit
CCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATC131570.11873936821486908No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTCTGTAGGCACCATCAATAGA131460.1186400953524868No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA129360.1167448861615525No Hit
GGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATCAATAGA129270.11666366291051247No Hit
CATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCA127760.115300917254174No Hit
TTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAAT125750.11348693131427974No Hit
TTGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGAT125610.11336058403488412No Hit
ATGGCGCTTAAGCGCGCGACCTATACCCGGCCTGTAGGCACCATCAATAG124070.11197076396153231No Hit
TCTACCGTTGGTGTTAAAGGGAGAACTGCGAGGAGCTGTAGGCACCATCA123130.11112243222844743No Hit
TTAGGATATCTGGCTTTCACCCAGGAGACCCGGGCTGTAGGCACCATCAA122300.11037337335774483No Hit
TCGAGGGCACGTCTGCCTGGGTGTCACACTGTAGGCACCATCAATAGATC121260.1094347935679488No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG121110.10929942148288206No Hit
AAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATCAATA119660.10799082466057029No Hit
GGAAGGTCACGGCGAGACGAGCCGTTTACTGTAGGCACCATCAATAGATC118220.10669125264392965No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG116340.10499458917775989No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATA115850.10455237369987522No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCCTGTAGGCACCATCA113960.10284668542803435No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCTGTAGGCACCATCA112650.1016644358851182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)