FastQCFastQC Report
Sat 2 Sept 2023
SRR12343636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12090276
Total Bases870.4 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA3570932.953555402705447No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT2040091.6873808339859238No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG1910801.580443655711416No Hit
AAAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCA1673081.3838228341520078No Hit
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAG1379001.1405860379035186No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA1127120.9322533249034183No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC954840.7897586457083362No Hit
TACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATA727190.6014668316918489No Hit
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA700010.5789859553247585No Hit
AGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAGA600390.4965891597511918No Hit
AAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCTGTAGGCACCATCA537700.4447375725748527No Hit
AAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAGATCGGAAG516900.4275336642438932No Hit
TAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA376390.3113163008023969No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT342070.2829298520563137No Hit
TCGGGGCTGAAGCCATGCACCGAAGCTGAGGACTGTAGGCACCATCAATA331580.27425345790286343No Hit
TAGACCCGGGTTCGTTTCCCGGCTGGTGCACCACTGTAGGCACCATCAAT312860.25876994040499984No Hit
AGGTCAGGCGGGATTACCCGCTGAGTCTGTAGGCACCATCAATAGATCGG270990.22413880377916928No Hit
ATCCGTGTGTCGACAGTTCGATTCTGTCCTGTAGGCACCATCAATAGATC262550.2171579871294915No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCTGTAGGCACCATCAATAGA259360.2145195031114261No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCATCAATAGAT247930.20506562463917283No Hit
ATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGAA244070.2018729762662159No Hit
ATCCTTGTGTCCCTGGTTCGATTCCTGTAGGCACCATCAATAGATCGGAA230340.19051674254582776No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG228810.18925126275032927No Hit
TAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAGATCGGAAG227900.1884985917608498No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA226840.18762185412475282No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA221730.18339531702998343No Hit
TATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCA217610.17998761980288952No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG199650.16513270664788796No Hit
AGTAAGCGCGAGTCATCAGCTCGCGTTGACTCTGTAGGCACCATCAATAG188840.15619163698165367No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCACTGTAGGCACCATCAATA186240.15404114844028374No Hit
AGACCCGGGTTCGTTTCCCGGCTGGTGCACCACTGTAGGCACCATCAATA183520.15179140658161983No Hit
TGTGGGGATGACGTCAAGTCCTCATGGCCCTCTGTAGGCACCATCAATAG164610.13615073799804076No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAAT160200.13250317858748634No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGCTGTAGGCACCATCAAT158290.13092339662055688No Hit
ACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATCAATAG157020.1298729656791954No Hit
TTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC155250.12840897924910893No Hit
AGAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCATCAATAG149520.1236696333483206No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA143950.11906262520392422No Hit
TCGGGGCTGAAGCCATGCACCGAAGCTGAGGCTGTAGGCACCATCAATAG142930.11821897200692524No Hit
AAATAGCAGCGCCGGGCAGCTAAGTTGGTACTGTAGGCACCATCAATAGA138100.11422402598584185No Hit
AGCTCTCTGGGTCCCTACCGACGCTGGGGCTGTAGGCACCATCAATAGAT134450.11120507091814942No Hit
CCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC129300.10694544938428205No Hit
TATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGA127290.10528295631960759No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG125020.10340541440079613No Hit
TAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCTGTAGGCACCATCAA124420.10290914781432615No Hit
CACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATA123690.10230535680078767No Hit
TAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCTGTAGGCACCATCA123440.10209857905642518No Hit
TCCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG122900.10165193912860218No Hit
TCGTGGGTTCGGGCCCCACGGTGGGCGCCACTGTAGGCACCATCAATAGA122140.10102333478574022No Hit
TAGGATACTCGGCTCTCACCCGAGAGACCCGGGTCTGTAGGCACCATCAA121910.1008330992609267No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC121780.10072557483385822No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGA121570.10055188152859372No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)