FastQCFastQC Report
Sat 2 Sept 2023
SRR12343637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47635580
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA9059231.9017780406998297No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT8667881.8196230632648958No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG4764361.0001683615482377No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3011070.6321052457007976Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG2301560.48315985656099913No Hit
AAAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCA2267240.47595515788828435No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG2158950.4532221503338471No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC2146020.45050779270452884No Hit
AGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAGATC2127320.4465821556072163No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA2105930.4420918145638197No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1805240.3789688296017389No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG1800240.37791919401422214No Hit
ATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAATAGATCGG1764970.37051506457987915No Hit
ACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCG1664400.3494026943725677No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA1621580.3404136152010745No Hit
GGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT1603150.3365446584254878No Hit
TGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT1523580.31984075768574666No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA1477850.3102407906023187No Hit
ATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAGATCGGA1473230.30927092731945327No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGA1365030.2865568132055913No Hit
ACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGG1325880.2783381665553353No Hit
GGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAATA1291010.2710180079679937No Hit
AAAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATCGGAAG1197300.25134573778675523No Hit
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAG1155870.2426484573085916No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAAT1114450.23395327610160307No Hit
AAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAAG1074050.22547222055446792No Hit
ATTAGACTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGATCGG1010920.21221952162648172No Hit
ATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGAA999520.20982635248694356No Hit
AGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT967830.20317376213326255No Hit
TAGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAA942220.1977975286540019No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA914350.19194685988918367No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATA898650.18865100414438116No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA895280.18794354975839486No Hit
ATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGATCGGA879490.18462880057301706No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAATA847390.1778901401011597No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT815580.17121235849337826No Hit
AAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGATCGGAA762960.16016599357035224No Hit
TATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAG745100.1564166952517425No Hit
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA730110.15326988776036737No Hit
CCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG703650.14771521623122885No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGA657430.13801238486022424No Hit
AGAGCACTGGGCAGAAATCACATTGCGTTAGCATCTGTAGGCACCATCAA637420.1338117432389823No Hit
GGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGAT610330.12812481762581668No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG609240.12789599706773802No Hit
TAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAG598400.12562038711400175No Hit
AAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAGA588940.12363447658242013No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA577660.12126649869698237No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA562680.11812179047678227No Hit
TAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAA560380.11763895810652457No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA552250.11593225064122237No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT533030.11189745144280809No Hit
TAGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATA531090.11149019283485159No Hit
CTCTAGGGATAACAGGCTGATCTTCCCCAAGCTGTAGGCACCATCAATAG530890.11144820741135093No Hit
TTGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCA526060.11043425943380977No Hit
TTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAA510660.10720138182425826No Hit
AAACTCGACGGATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC509790.10701874523203034No Hit
GAGTAGCATGGGGCACGTGGAATCCCGTGTGAACTGTAGGCACCATCAAT497310.10439885480558858No Hit
TAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAGAT497230.10438206063618832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)