FastQCFastQC Report
Sat 2 Sept 2023
SRR12343642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14310990
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6295764.3992484097885605Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT2338541.6340868102067012No Hit
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAG1274320.8904485294169027No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1266500.884984197459435No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA1139670.796360000251555No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT895910.6260293662423075No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA766110.5353298409124735No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG617200.43127694170703773No Hit
ACTCCGCTAGTAGTGGCGAGCGAACGCGGACCTGTAGGCACCATCAATAG598670.4183288507643427No Hit
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA521770.3645939239703193No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA520900.3639859995709591No Hit
AGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAGA507390.35454570228894017No Hit
ATCCGTGTGTCGACAGTTCGATTCTGTCCTGTAGGCACCATCAATAGATC445840.3115367979433987No Hit
TAGGGCGGGGCACGTGAAACCCTGTCTGACTGTAGGCACCATCAATAGAT415440.29029438214966263No Hit
ATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGAA406670.2841662246986407No Hit
AGGTCAGGCGGGATTACCCGCTGAGTCTGTAGGCACCATCAATAGATCGG383620.2680597219339822No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG377000.26343390638942515No Hit
TAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA374600.26175687356360394No Hit
TCGGGGCTCAAGCCGTGTACCGAAGCTGCGGACTGTAGGCACCATCAATA359740.2513732453170605No Hit
AGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAGATC353540.2470409105170222No Hit
TATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAG346540.24214956477504349No Hit
ATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGATCGGAAG342190.2391099427782425No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA328020.2292084614691227No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG306980.2145064736960895No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG304480.21275956450252567No Hit
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA298720.2087346857205546No Hit
AGTAGGGCGGGGCACGTGAAACCCTGTCTGACTGTAGGCACCATCAATAG277520.19392089575913338No Hit
ATCCTTGTGTCCCTGGTTCGATTCCTGTAGGCACCATCAATAGATCGGAA262650.18353027987581572No Hit
ATTAGCTGATGATTCATGATAACTCTGTAGGCACCATCAATAGATCGGAA258990.18097280481643827No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA252830.17666842056349702No Hit
AGGGCGGGGCACGTGAAACCCTGTCTGACTGTAGGCACCATCAATAGATC235820.16478245041048872No Hit
ACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGG233450.1631263804949902No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC215820.15080717686197812Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGTCAGGCGGGATTACCCGCTGAGCTGTAGGCACCATCAATAGATCGGA212300.14834752871744022No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA211440.1477465919548543No Hit
TTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC193520.13522474685538877No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG193230.13502210538893536No Hit
CATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT190720.13326820855859728No Hit
TATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGA182450.12748943294628814No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCTGTAGGCACCATCAATAG169140.11818888839975432No Hit
TATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGATCGGAA167810.11725953270877836No Hit
AAAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCA167210.11684027450232304No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA165090.11535889550618092No Hit
ACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATCAATAG162010.11320670337971028No Hit
GATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT161230.11266166771131836No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG160190.1119349534867958No Hit
ACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA155070.10835728345837708No Hit
TACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA147870.10332618498091327No Hit
TAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAGAT146900.1026483842138105No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC145620.10175396670670583Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)