FastQCFastQC Report
Sat 2 Sept 2023
SRR12343644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113944511
Total Bases13.1 Gbp
Sequences flagged as poor quality0
Sequence length72-122
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA14988051.3153814842384113Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT14817731.3004338576695458No Hit
ATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCGGAAGA14792571.2982257653464324No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA8157430.7159125023582751No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC6857750.6018499653748131No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA6637110.5824861541597208No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA5978060.5246465975004272No Hit
ACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCG5525660.48494306145207816No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC5445570.4779142015888769No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG4871720.427551968694657No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAAT4177240.3666030038077043No Hit
AGCTCTCTGGGTCCCTACCGACGCTGCTGTAGGCACCATCAATAGATCGG4031510.35381344521281943No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATA4025410.35327809691508527No Hit
ATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGATCGGA3947630.34645196730889477No Hit
ATTCAGCCCTTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAGATCG3541510.31081005736204353No Hit
ACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAATAG3285190.28831489741528665No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA3283480.28816482436788904No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG3103380.2723588852823283No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG2902610.2547389053255931No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA2585800.22693502102966592No Hit
TATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCGGAAG2578150.2262636416070977No Hit
TGAGATTCAGCCCTTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAG2458720.21578222403359124No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA2389650.20972050158695227No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA2378710.20876038513167167No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG2271780.19937599275844012No Hit
ACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGG2249180.1973925711963431No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTACTGTAGGCACCATCAATAGATC2220340.19486151465426887No Hit
TTTTGATCCTTCGATGTCGGCTCTTCCTACTGTAGGCACCATCAATAGAT2219880.19482114412689874No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG2210710.19401636643997708No Hit
AAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCAA2122530.18627751186715788No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCATCAATAGAT2102920.1845564987329666No Hit
CCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC1765600.1549526154884284No Hit
TGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT1680360.14747178124271385No Hit
ATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGAT1627350.14281951677338806No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTATCTGTAGGCACCATCAATAGAT1616910.14190328132611846No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA1600560.1404683723641589No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGA1600170.1404341451779103No Hit
AGAAATCACATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAGA1539680.13512542082874004No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA1532560.13450055527466348No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAGA1526510.13396959507773043No Hit
ACCGGGACGTGGCGGTTGACGGCAACGTTCTGTAGGCACCATCAATAGAT1469080.12892942249758743No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACTGTAGGCACCATCAAT1450570.12730494758102037No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA1426890.12522674304161963No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG1422320.12482567062839912No Hit
TAGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAA1407440.12351977182999188No Hit
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA1405820.12337759736403625No Hit
ACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA1394850.12241484804827502No Hit
TATACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGA1382160.12130114806495594No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT1364390.11974161704024515No Hit
AGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAGA1317760.11564927423313967No Hit
TTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCGGAAGAG1288710.11309978766770082No Hit
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAATA1274830.11188165088531557No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA1271620.11159993481388499No Hit
TTGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCA1269680.11142967650280232No Hit
AAAACTCAGCGGGTAATCCCGCCTGACCTGTAGGCACCATCAATAGATCG1240260.10884771799143532No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG1239130.10874854691333047No Hit
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAG1218320.10692221935991283No Hit
ACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATCAATAG1182030.10373733579847476No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)