FastQCFastQC Report
Sat 2 Sept 2023
SRR12343648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54860545
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC13752802.506865361982824No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA4887870.8909627128202974No Hit
TCGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTACTGTAGGCACCAT4524300.824691041621989No Hit
TCGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGCTGTAGGCACCATCAAT4160720.7584175476200611No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGA4096950.7467935289377822No Hit
TCGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA3890940.7092419515701129No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG3827250.697632515316791No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC3320430.6052491822675112No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC3097070.5645350406198115No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATA2967890.5409880634616371No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG2828030.5154943320377149No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2731310.49786417542880773Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAATAG2416310.44044586141096487No Hit
TAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACC1941160.3538353474250028No Hit
TTCGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCAT1865660.34007318009691667No Hit
CCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGCTGTAGGCACCATCAA1720500.31361336275459895No Hit
TCGGAGGAGCACGAAGGTTGGCTAATCCTGGTCTGTAGGCACCATCAATA1698310.30956856152267537No Hit
TTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAATA1682710.30672498787607744No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCTGTAGGCACCATCAATAG1656100.3018745074442844No Hit
TGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT1649270.300629532572088No Hit
TTTGTCACTGCTTATGGACCCGAACCTGGGTGCTGTAGGCACCATCAATA1613770.2941585797224581No Hit
TTTGCAGTTAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAG1565470.2853544382397222No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCAT1551100.28273506943833676No Hit
TAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAATAG1549340.2824142560012847No Hit
TTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATA1523870.2777715751821277No Hit
GGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT1476600.26915518247221204No Hit
AAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCTGTAGGCACCATCA1441630.26278083821442166No Hit
TTTGGGCCAACCTATGGTGTACCGGTTGTTATCTGTAGGCACCATCAATA1431790.2609871994527214No Hit
GCGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC1431300.26089788207536035No Hit
TAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGCTGTAGGCACCA1386990.25282103923685045No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAAT1369500.24963295570614547No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA1262900.2302018691210596No Hit
TATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAG1260000.2296732560713715No Hit
CCGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC1248910.2276517668572195No Hit
AAAGAATTCCACGTTGTGGCCGTGGAGACCTGGGCTGTAGGCACCATCAA1245020.22694269624918967No Hit
ATGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA1149660.20956044093254997No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCCTGTAGGCACCATCAATA1149370.20950757962758118No Hit
TAAGAATTCCACGTTGTGGCCGTGGAGACCTGGGCTGTAGGCACCATCAA1138350.20749885003876647No Hit
TTTGGGCCAACCTATGGTGTACCGGTTGTTACTGTAGGCACCATCAATAG1133430.20660203065791635No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC1082860.19738411275352805No Hit
ACCGGGAAGGACGCACCTCTGGTGTACCAGTCTGTAGGCACCATCAATAG1075830.1961026818089394No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1062930.19375126513963722No Hit
AAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTCTGTAGGCACCATCA1010690.1842289390307734No Hit
AAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCAC1002940.1828162662255725No Hit
AGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAATAGAT1001260.18251003521747733No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCAT955890.17423997519528833No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAAT952320.17358923430308612No Hit
CGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATCA919750.16765236291400315No Hit
ATGTACACGGAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA861190.1569780249175432No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCATC830450.15137472659084958No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACC829320.1511687497818332No Hit
AAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCTGTAGGCACCATC821670.14977430501282843No Hit
TGCACCAGTGGTCTAGTGGTAGAATAGTACCCTCTGTAGGCACCATCAAT799000.14564200920716336No Hit
GGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCAT796110.1451152189610949No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG779580.14210212457787286No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCA777660.14175214628290697No Hit
TGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATCA775700.1413948767734626No Hit
TCAGGATACTCGGCTCTCACCCGAGAGACCCGGGTTCTGTAGGCACCATC765580.1395501995104132No Hit
ACGGAGGAGCACGAAGGTTGGCTAATCCTGGTCTGTAGGCACCATCAATA764250.1393077666290045No Hit
GCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATC763100.13909814421274888No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA755620.13773468710527756No Hit
GGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCA748020.13634935635437087No Hit
TGCACCAGTGGTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATA726750.1324722530554518No Hit
ATTGTCACTGCTTATGGACCCGAACCTGGGTGCTGTAGGCACCATCAATA707660.12899252094560854No Hit
GCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAG704380.1283946413583751No Hit
ACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGCTGTAGGCACCATCAAT702750.12809752436837804No Hit
ACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTCTGTAGGCACCATCAAT692300.12619269458588134No Hit
TAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCCTGTAGGCACCAT661290.12054018056145814No Hit
TTGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAA658050.11994959218870319No Hit
AGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCTGTAGGCACCATCAAT645800.11771665775467596No Hit
TTAATACCCCGTAGGCTGAGGAGCAAAAGGACTGTAGGCACCATCAATAG637310.11616909748162363No Hit
GGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATCA623890.11372289502410157No Hit
TGACCGGGAAGGACGCACCTCTGGTGTACCAGTCTGTAGGCACCATCAAT615880.11226282932479069No Hit
AGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCA614910.11208601737368815No Hit
CCCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGCTGTAGGCACCATCA607710.11077359876756601No Hit
GACCGGGAAGGACGCACCTCTGGTGTACCAGTCTGTAGGCACCATCAATA604680.11022128927082296No Hit
TTAGAATCCCACGTTGTGGCCGTGGGGACCTGGGCCTGTAGGCACCATCA603090.10993146349530432No Hit
TTTGCCAAGGATGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATAG588580.10728657544324433No Hit
TGCACCAGTGGTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAG580810.1058702570308042No Hit
AAACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAA573090.10446305263646213No Hit
TTAGAATCCCACGTTGTGGCCGTGGGGACCTGGGCTGTAGGCACCATCAA554750.10112003079808995No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA552950.10079192614655944No Hit
TCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATC552020.10062240540993532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)