FastQCFastQC Report
Sat 2 Sept 2023
SRR12343649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20309613
Total Bases442.6 Mbp
Sequences flagged as poor quality0
Sequence length11-56
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGTGGCGAGCGAACGCGGACC4431852.1821439926009423No Hit
AGTGGCGCGAAGCTACCGTGCGCTGG4131202.034110645042818No Hit
GAAGCCATGCACCGAAGCTGAGGA4124282.0307033915417296No Hit
GTCGGCGGTTCGATTCCGTCC2403761.183557756615057No Hit
CAGTGGCGCGAAGCTACCGTGCGCTGG2237671.1017787488122004No Hit
GGGGCACGTGGAATCCCGTGTGA2192481.0795282017436767No Hit
GTGGCGCGAAGCTACCGTGCGCTGG1994350.9819734132797113No Hit
AGTAGTGGCGAGCGAACGCGGAC1957820.9639868568642839No Hit
GAAGCCATGCACCGAAGCTGAGGAT1728730.8511880556266631No Hit
AAGTAGTGGCGAGCGAACGCGGACC959410.47239206379757215No Hit
GCCCGAGGTAGAGCACTCAATGGGCT765560.37694465177647646No Hit
ATTGGGACCGAAACGGTCTCAACCT731810.36032690529356715No Hit
ACCCACCTGAAAACGAGTGTTCCC616640.30361976862877693No Hit
GCCCGAGGTAGAGCACTCAATGGGC555600.27356503543420546No Hit
GGCCCGAGGTAGAGCACTCAATGGGCT522630.2573313435366789No Hit
GCCGCACTCACGAGGGACTGCCAG521600.25682419453290417No Hit
AAAGATAACTCTTC507190.24972903225679385No Hit
CTCACCCGAGAGACCCGGGT488360.24045756066351434No Hit
TTCATGATAACTCGACGGATCGC472340.23256967033296005No Hit
CCCACCTGAAAACGAGTGTTCCC450500.22181614194224183No Hit
GGGGCACGTGAAACCCTGTCTGA447910.22054088376770153No Hit
TCGTAGTTGAACCTT424400.2089650846621253No Hit
GCACGTGGAATCCCGTGTGA424180.2088567615739404No Hit
AAGTAGTGGCGAGCGAACGCGGAC416230.20494235906907732No Hit
GGCCCGAGGTAGAGCACTCAATGGGC401400.19764039817006854No Hit
TTGGGACCGAAACGGTCTCAACCT398410.19616818892610113No Hit
GCCCGAGGTAGAGCACTCAATGGGCTA376910.18558206894439594No Hit
GTCGACAGTTCGATTCTGTC355980.1752766042366243No Hit
CAACACCGTGGAAGTTCGAGTCTTCTC353510.17406043138291213No Hit
CCCGAGGTAGAGCACTCAATGGGCTA336610.16573924869961826No Hit
TGCCGCACTCACGAGGGACTGCCAG334920.16490713043128888No Hit
GCGGGTAATCCCGCCTGAC333990.16444921919487093No Hit
TTTGGTGGATGCCTTGGC329300.16213996790583848No Hit
GGATGCCCGGGCATTGA329140.16206118747806766No Hit
GCGAGAGAACTCTCGTTAAGGAA325570.16030339918343103No Hit
GCCGCACTCACGAGGGACTGCCA325510.16027385652301696No Hit
GATGCCCGGGCATTGA311690.15346919707431156No Hit
GTGGAGCATGTGGTTTAATTCG301490.14844694480392118No Hit
AAGCGATCTGCCGAAGCTGTGGG299740.14758528387517775No Hit
GAAGCCATGCACCGAAGCTGAGG291130.14334591210575998No Hit
CAAGTGATTCACCGAAGCGACGAGAC290760.14316373236653993No Hit
TGGGGCACGTGGAATCCCGTGTGA290140.14285845820892795No Hit
AACCCTTGTCCTTAGTTACCAG280670.13819564164024198No Hit
GGAGCTGGAATTACCGCGG279820.13777712061770947No Hit
CCTTGAAAATCCGGAGGACCG273810.13481793079956766No Hit
GGCACGTGAAACCCTGTCTGA267680.1317996556605978No Hit
GAGAGAACTCGGGTGAAGGAA266740.13133682064744415No Hit
AACCCACCTGAAAACGAGTGTTCCC263590.12978583097570595No Hit
TTGGATGGATGCCCGGGCATTGA261550.12878138052162788No Hit
CAACCCTTGTCCTTAGTTACCAG254220.12517225217437675No Hit
TTAGCTGATGATTCATGATAACTC251560.1238625275626867No Hit
TGGGACCGAAACGGTCTCAACCT250460.12332091212176224No Hit
GGCCCGAGGTAGAGCACTCAATGGGCTA249680.12293685753637945No Hit
CGCGTGAGTGATGAAGGCCTT241140.11873195220411141No Hit
CCCTTGAAAATCCGGAGGACCG238960.11765856887573387No Hit
CAACCCTCGCCCTTAGTTGCCAG235930.11616666452482378No Hit
GGAGGAAAAGAAACT235500.11595494212518968No Hit
GGCACGTGGAATCCCGTGTGA235110.11576291483249829No Hit
TGGAGCATGTGGTTTAATTCG234220.11532469870302305No Hit
GCGAGAGAACTCTCGTT232140.11430055314200227No Hit
TCGTAGTTGAACCTTG227320.11192729275540601No Hit
GCCGCACTCACGAGGGACTGCCAGT219720.10818522243629161No Hit
TGCCGCACTCACGAGGGACTGCCA218310.1074909699165612No Hit
CTGGAAAGGGGCGCC215730.10622063551875656No Hit
TGGAATTACCGCGG212460.10461056052619024No Hit
CCGGCGGCCCTTGAAAATCCGGAGGACCGA212450.10460563674945456No Hit
CTTGGTTGAAAAGCCAGTGGCGCGAAGC205960.10141010564800029No Hit
AAAAAGCTCGTAGTTGAACCTT205350.101109755267124No Hit
GCAACCCTCGCCCTTAGTTGCCAG204580.10073062445847689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)