FastQCFastQC Report
Sat 2 Sept 2023
SRR12343650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37459282
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9449862.5227018499713902Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC5044731.3467236238003708No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA4063181.084692440180781No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG4035011.0771722746848165No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC2762190.7373846620979013No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG2143810.5723040820696991No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG1992970.5320363588389121No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG1731560.4622512519060029No Hit
GTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA1678250.4480197992049073No Hit
TGTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAG1543920.41215952831130076No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT1522800.4065214063633147No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA1506590.4021940409856227No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG1422880.37984710972303204No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG1416310.37809320531023527No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA1250310.33377842105996586No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA1237980.33048684702499104No Hit
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT1181740.31547321168622505No Hit
TATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAG1150950.307253620077395No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG1060450.28309405396504933No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG1037700.27702079287050935No Hit
AAAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAAT1001840.2674477316463247No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC956070.2552291311937052No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT911390.2433015133605604No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA866620.2313498694395691No Hit
ATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGATCGGA857330.22886984326074378No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT847020.22611752141965774No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCA802350.21419257315182924No Hit
TAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAG774090.20664838156801832No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG763790.20389872929224856No Hit
TTGAAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAAT727800.19429096371895221No Hit
ATTTGGTGGATGCCTTGGCATGCACCTGTAGGCACCATCAATAGATCGGA718440.19179225058291294No Hit
AAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAGA714420.19071908532576784No Hit
AGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAACCTGTAGGCACCATCA704750.18813761566492385No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAATA684770.18280382416299384No Hit
ACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGG683820.1825502154579471No Hit
AGTAGGACGGCGCACGTGAAACGTTGTCTGACTGTAGGCACCATCAATAG662620.17689073698743077No Hit
TAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAA657550.17553726737207617No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA651200.17384209339623755No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG649830.173476362947907No Hit
TAGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAACCTGTAGGCACCATC639210.17064128458201627No Hit
AAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAAG579360.15466393616407276No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA576200.15382035352412787No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATA573260.1530355013211412No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA567390.15146846648048407No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA567360.1514604577845352No Hit
TGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGAT548730.14648705760030317No Hit
AAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATAG530880.14172188351074108No Hit
TCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGATC519030.13855844861094777No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAG514610.1373785007411514No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGCTGTAGGCACCATCAATAG505300.13489313543169354No Hit
AACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATA503780.13448736150361879No Hit
AAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGA482070.12869173520197208No Hit
TAGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAA467480.12479683940551771No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCCTGTAGGCACCATCAATAGAT452410.1207738044738818No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA441390.1178319434953398No Hit
AAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAG439400.11730069999739984No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCAA422300.11273574330655885No Hit
AGGGCGGGGCACGTGAAACCCTGTCTGAACCTGTAGGCACCATCAATAGA422110.11268502156554949No Hit
TGAGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAAT414480.11064814322922686No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACTGTAGGCACCATCAAT413680.11043457800392438No Hit
TGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAAT411270.10979121276270057No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT395580.10560266478145523No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC389620.10401160385295159Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATA385580.10293309946517394No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTGTAGGCACCATCAATA385520.10291708207327627No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCTGTAGGCACCATCA383430.10235914292217346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)