FastQCFastQC Report
Sat 2 Sept 2023
SRR12343657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217186036
Total Bases25.3 Gbp
Sequences flagged as poor quality0
Sequence length72-122
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT99689434.590047861088086No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC33016891.5202123768215008No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGA31235591.4381951333187921No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG27590501.2703625199918471No Hit
AAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAAG24371301.1221393625877494No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG20718030.9539301136284839No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA17616600.8111294963733303No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA16461660.757952044393867No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA11643430.5361039878272837No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGA11580190.5331921984155555No Hit
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAG10305940.4745212993343642No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG9464320.43577018920314015No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC8977390.4133502395153987No Hit
AGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCG8766220.40362723872357986No Hit
TCGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGCTGTAGGCACCATCAAT7921970.3647550342509129No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG7362060.33897483169682235No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA6746880.31064980623339894No Hit
AAGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAG6396520.29451801404027655No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT5681630.2616019936014671No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG5421830.2496398985798516No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5154080.23731175792535758Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAA4961090.2284258275241968No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG4645640.21390141307243163No Hit
AAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAGA4638740.21358371308917856No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTCTGTAGGCACCATCAAT4576030.21069632671964234No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAATA4501070.20724490777114232No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG4410390.20306968538253536No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG4316930.19876646213111052No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGTAGGCACCATCAATAG4113640.1894062839288618No Hit
ATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGATCGGA4100820.18881600656867276No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA3984040.1834390494608042No Hit
TGCGAGAGGTCCCGAGTTCATTTCTCGGAACGCCCCTGTAGGCACCATCA3758710.17306407305117905No Hit
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA3437290.15826477904868616No Hit
ACTATGCCTGAGCGGGGTAAAGCCAGAGCTGTAGGCACCATCAATAGATC3134880.14434077152179342No Hit
TGCGAGAGGTCCCGAGTTCATTTCTCGGAACGCCCTGTAGGCACCATCAA3114890.14342036243987621No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA2940700.13540004938439043No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGCTGTAGGCACCATCA2880960.13264941213808054No Hit
TCGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGCTGTAGGCACCATCAATA2852460.1313371730768179No Hit
GTCGTTGTAGTATAGTGGTAAGTATTCCCGCCTGTAGGCACCATCAATAG2823690.13001250227708008No Hit
GCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAG2814050.1295686431700425No Hit
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA2808390.1293080370968233No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT2766340.12737190893801292No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA2756110.12690088418023338No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA2671290.12299547656001236No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA2518230.11594806214889433No Hit
TGCGAGAGGTCCCGAGTTCATTTCTCGGAATGCCCTGTAGGCACCATCAA2509640.11555254869148217No Hit
TTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGG2500770.11514414306083656No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATA2471430.11379322747987351No Hit
AGGTAGCCGTACTGGAAGGTGCGGCTGGATCACCTGTAGGCACCATCAAT2381060.10963227856877501No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG2358080.1085741994941148No Hit
CCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGCTGTAGGCACCATCAA2356350.10849454428092237No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGCTGTAGGCACCATCAATAG2351600.10827583777071194No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA2328850.10722834869549347No Hit
TAAGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATA2313210.1065082287334532No Hit
TCCGTTATCGTCCAGCGGTTAGGATATCTGGCCTGTAGGCACCATCAATA2303600.1060657509306906No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAAT2298410.10582678529111329No Hit
AGTTAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGG2290250.10545107052830965No Hit
ATCAGAAGGTTGCGTGTTCGTTTCACGTCGGGCTGTAGGCACCATCAATA2285130.10521532793204072No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA2251820.10368161975201756No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCGGCTGTAGGCACCATCAATA2246240.1034246971568651No Hit
TAATACCGTATGTGCCCTTCGGGGGAAAGATTCTGTAGGCACCATCAATA2182510.10049034644197842No Hit
CTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAGA2179590.1003558994925438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)