FastQCFastQC Report
Sat 2 Sept 2023
SRR12343661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45212055
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length72-122
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA3427470.7580876383522049No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAAT3153980.6975971342156423No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA2970180.6569442596670291No Hit
AAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCAA2858110.6321566228299068No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAAT2230270.49329100391477454No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCAT2077020.4593951767952154No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA2069850.457809316563912No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT2054830.4544871937362724No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC1923440.42542636029262554No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA1748230.3866734215022962No Hit
TCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATC1723700.381247877363681No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA1601030.3541157330716332No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT1595320.35285279556525356No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG1451630.3210714487540989No Hit
TAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCA1355930.2999045276751963No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG1252590.2770477917891589No Hit
GCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATC1233010.2727170883960041No Hit
GCCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAA1217390.26926225759921774No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1184730.2620385204786644No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACTGTAGGCACCATCAAT1142300.2526538552605052No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTTCTGTAGGCACCATCAATAG1070890.23685939513255921No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGCTGTAGGCACCATCAATA1065840.23574243639224093No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG1042440.2305668255955187No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG991620.21932646060879116No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA988350.21860320217694154No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG962600.21290781850106127No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACC933240.20641397521081492No Hit
TAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACC924580.20449855685613053No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA913980.20215404940120504No Hit
TTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCCCTGTAGGCACCATC878960.1944083276020079No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA874580.19343955942723684No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA827800.1830927614327639No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT824310.18232084341222712No Hit
TTGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCA818990.18114416608579284No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAATA818150.18095837492898742No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA755230.16704173256446758No Hit
AAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCA750080.16590265582929153No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA746120.16502678323292316No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG732470.1620076769348352No Hit
TTTTGATCCTTCGATGTCGGCTCTTCCTACTGTAGGCACCATCAATAGAT697030.15416905955723534No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTCTGTAGGCACCATCAATAGA688160.1522071934133496No Hit
TTAGGATATCTGGCTTTCACCCAGGAGACCCGGGCTGTAGGCACCATCAA684800.15146402878612797No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT677090.14975873138259255No Hit
AAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCAC646050.14289330577873532No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGA639520.14144900071452182No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTCTGTAGGCACCATC633850.14019491040608528No Hit
ACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTGTAGGCACCATCAATAG618390.13677546840107135No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAG609660.13484456744998652No Hit
TCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAACTGTAGGCACCATCA604300.1336590429256091No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATA599380.13257083757860597No Hit
AGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCA598910.13246688300277437No Hit
TCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAAT586940.12981935901829722No Hit
CCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC586910.12981272361983986No Hit
CTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAATA584050.1291801489669072No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG579660.1282091689926503No Hit
GCATTTATTAGACTGATGATTCATGATAACTCCTGTAGGCACCATCAATA573060.12674938133203634No Hit
AAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGCTGTAGGCACCAT571720.12645300020094197No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA556860.12316626616507478No Hit
TCGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGCTGTAGGCACCATCAAT550270.1217086903039466No Hit
TTAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATC518520.11468622693659909No Hit
GGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCAT515910.11410894727081083No Hit
TCGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGCTGTAGGCACCATC514290.1137506357541147No Hit
CTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAATA513800.11364225757931154No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGCTGTAGGCACCATCA513360.11354493840193727No Hit
TCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCA509270.11264031241225378No Hit
TTGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATC504710.11163173184673868No Hit
TGAGATTCAGCCCTTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAG504480.11158086045856576No Hit
TATCGGATGATATAGCCTCGAATCTGAGCTCTCTGTAGGCACCATCAATA501550.11093280320923259No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAGA498240.11020069757943982No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG493390.10912797482883713No Hit
CGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATCA487600.10784734292657125No Hit
TCGCATTTATTAGCTGATGATTCATGATAACTCGCTGTAGGCACCATCAA485370.10735411164124259No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAAT480960.10637870806801417No Hit
ATCGGATGATATAGCCTCGAATCTGAGCTCTCTGTAGGCACCATCAATAG472130.10442568912207154No Hit
TTCAGTTGATGTATGCCACACCTCTGAGCTCTCTGTAGGCACCATCAATA454820.10059706421218853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)