FastQCFastQC Report
Sat 2 Sept 2023
SRR12343663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89745084
Total Bases10.4 Gbp
Sequences flagged as poor quality0
Sequence length72-122
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA12694911.4145521330171134Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA6388710.7118729756829912No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA5569500.6205910955523758No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAAT5140600.57280017699911No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAAT4418690.49236011634910276No Hit
AAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCAA4340430.48363986154383676No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT4182820.4660778968127101No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC4019270.44785405738770045No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC3916410.4363927053653435No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA3665670.4084535705599206No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA3515510.3917217348640511No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA3427790.3819473833240827No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG3383680.377032350875063No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT3266100.36393079759109703No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG2660570.2964585781656854No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACTGTAGGCACCATCAAT2252870.25102990599462804No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGCTGTAGGCACCATCAATA2246120.25027777566067017No Hit
GCCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAA2183030.24324786413927696No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG2140710.23853228551215128No Hit
TCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATC2030000.2261962337680803No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG2022990.2254151324879255No Hit
ACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGG1978830.22049452870309866No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA1908110.21261443133754268No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA1823750.20321447356381103No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA1689610.18826769386053502No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAATA1665030.18552882517776684No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCAT1627110.18130352410166556No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG1543790.1720194501127215No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG1515160.16882930322957856No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT1460030.162686348368675No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA1449010.16145842595679114No Hit
GCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATC1430170.1593591466246775No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT1385850.15442071456526799No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGA1375770.1532975332665575No Hit
TTGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCA1296490.14446362321082679No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAG1260720.1404778895744306No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA1256420.1399987546950204No Hit
CCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC1217760.1356909978489741No Hit
ACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA1183320.13185346174504667No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAGA1174400.13085953543706083No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA1165000.12981212430532685No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG1161210.129389817051149No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATA1153190.12849617478769088No Hit
TCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAAT1142480.127302794657811No Hit
TCGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGCTGTAGGCACCATCAAT1127810.12566816473200915No Hit
TTAGGATATCTGGCTTTCACCCAGGAGACCCGGGCTGTAGGCACCATCAA1106880.12333600356315896No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG1020610.11372322076159626No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGCTGTAGGCACCATCA1013960.11298223309925254No Hit
TGAGATTCAGCCCTTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAG991110.11043613263540986No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAAT947200.10554338552961853No Hit
TCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAACTGTAGGCACCATCA924410.10300397066874438No Hit
TTCAGTTGATGTATGCCACACCTCTGAGCTCTCTGTAGGCACCATCAATA899110.10018487475035401No Hit
AAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCA897650.10002219174478683No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)