FastQCFastQC Report
Sat 2 Sept 2023
SRR12343668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36059846
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGGGCTCAAACCACACACCGAAGCTACGGGCTGTAGGCACCATCAATA6309321.7496802398989726No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG4756731.3191209968007074No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG3425040.9498210280764927No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG3021440.8378959799218221No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCAT2382380.6606739252297417No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG2374730.6585524519433611No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC1697930.4708644623718027No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACC1635680.4536014934728229No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA1616740.44834911385922166No Hit
ACGGGGCTCAAACCACACACCGAAGCTACGGGCTGTAGGCACCATCAATA1551490.4302541946518574No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGA1517690.42088088784405786No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG1492920.414011751464496No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG1454240.4032851388217243No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1429220.396346673249797Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA1424000.39489907971320787No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATA1211680.33601918322113744No Hit
ATGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA1198250.3322948190072692No Hit
ACGAGCGCAACCCTTGTCCTTAGTTACCAGCTGTAGGCACCATCAATAGA1103470.3060107355977061No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAATA1058100.2934288737672368No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG1048380.29073335476807083No Hit
TCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGATC948390.2630044509896132No Hit
GCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATC913000.25319021051836993No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAAT900520.24972929723549014No Hit
TCTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAATA845060.2343493092011541No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA843460.23390560237001568No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA818970.22711411468590298No Hit
GTCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGAT809910.2246016247545816No Hit
TCGGGAAACTAGCCTCAAGATGAGATCTCACCTGTAGGCACCATCAATAG787670.21843409980175735No Hit
CGGGGCTCAAACCACACACCGAAGCTACGGGCTGTAGGCACCATCAATAG755160.20941853162656324No Hit
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT715800.1985033435805577No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCA696530.19315944943303417No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC696260.19308457390527958No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC688020.19079948372491665No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCATC682700.18932415851138132No Hit
TAATACCGCATACGTCCTACGGGAGAAAGTGGGGGCTGTAGGCACCATCA660620.1832010042416709No Hit
GGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAGA639250.17727474487827818No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATA625650.17350323681360147No Hit
ACCCTGGTAGTCCACGCCGTAAACGATGTCGACTAGCTGTAGGCACCATC622010.17249380377276155No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTCTGTAGGCACCATCAAT599110.1661432497520927No Hit
TCGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAG595030.16501179733268967No Hit
CCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG559780.155236381209171No Hit
AAGAAGCGCATACGGTGGATGCCTTGGCAGTCACTGTAGGCACCATCAAT559270.1550949496567456No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCAT541120.15006165029101898No Hit
CCGGGGCTCAAACCACACACCGAAGCTACGGGCTGTAGGCACCATCAATA538020.14920196830568827No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA531660.14743823365191297No Hit
CGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAGA528400.14653418098346843No Hit
GCGGGGCTCAAACCACACACCGAAGCTACGGGCTGTAGGCACCATCAATA526430.14598786694762922No Hit
TCGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG511010.14171164236253256No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA510460.14155911813932873No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA505170.14009211242887726No Hit
TATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATA476870.13224404785311616No Hit
AGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACC476640.13218026499614002No Hit
CTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAGA475600.13189185555590005No Hit
GTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAGAT472090.1309184736950901No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACCTGTAGGCACCATCAATAG469840.13029451096380168No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAAT469070.1300809770513163No Hit
TATGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACC468500.12992290649272323No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTTCTGTAGGCACCATCAATAG439020.12174760812899756No Hit
TCGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGCTGTAGGCACCATCAAT429020.11897444043438234No Hit
AAACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAAT426470.11826728267225545No Hit
ATGGCGCTTAAGCGCGCGACCTATACCCGGCCTGTAGGCACCATCAATAG416160.11540814677910714No Hit
CTCTAGGGATAACAGGCTGATCTTCCCCAAGCTGTAGGCACCATCAATAG415550.11523898354973562No Hit
AGTAGGACGGAGCACGAGAAACTTTGTCTGACTGTAGGCACCATCAATAG414090.11483410106632182No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT411510.11411862380111108No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT398720.1105717423196982No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA394540.10941255822334904No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCCTGTAGGCACCATCAATAGAT390770.1083670740024791No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG390180.10820345710849681No Hit
TGTCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGA377560.10470371947789239No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCAA376180.10432102233603549No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG363550.10081851153773645No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAGA361960.10037757787429265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)