FastQCFastQC Report
Sat 2 Sept 2023
SRR12343669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32708966
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCGGAAGA12126863.7075033188147857No Hit
ACATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG9201072.813011576092011No Hit
ACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCG8712912.663768093433464No Hit
ATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC7343572.24512447137583No Hit
AAAAGTAACAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC5984131.8295075423662124No Hit
AGAAAAGATAACTCTTCCTGAGGCACCATCAATAGATCGGAAGAGCACAC5347091.6347474878906292No Hit
ACTTGGCTACCCAGCGTTTACTGTAGGCACCATCAATAGATCGGAAGAGC4524931.3833913306828471No Hit
TTGACGGCAACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4374201.3373091647103734No Hit
TGACGGCAACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3319561.0148776943911955No Hit
AAAAAGTAACAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2497490.7635490525747589No Hit
AAAACAGAAAAGATAACTCTTCCTGAGGCACCATCAATAGATCGGAAGAG2121020.6484521705761044No Hit
ACCTGTGTCCGCGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1919080.586713746927983No Hit
AAATACGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1889910.5777957028663028Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1844860.5640227208649763No Hit
ATTACTCCGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1811480.5538175679414629Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGCGTTAGCATCCGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1666310.5094352417010064No Hit
ACGTCAGTATCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1535750.46951958065565264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAATCACATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAGA1487230.45468572745466795No Hit
ACATTGCGTTAGCATCCGTAGGCACCATCAATAGATCGGAAGAGCACACG1432140.43784325068545427No Hit
ACCGTGCGCTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1426410.43609143743645096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAGGGACTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1351820.4132872925423568No Hit
AGGAGCGTATCGCCGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1318860.40321054477845614No Hit
TAGAAAAGATAACTCTTCCTGAGGCACCATCAATAGATCGGAAGAGCACA1250670.3823630499356048No Hit
TTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCGGAAGAG1250470.3823019046215035No Hit
AGACGAACGATTTGCACGTCAGTATCGCTGTAGGCACCATCAATAGATCG1147460.35080901059360914No Hit
AGGAGCGTATCGCCGACCGTAGGCACCATCAATAGATCGGAAGAGCACAC1102800.33715526195478024No Hit
AGCGGGTAATCCCGCCTGACCTGTAGGCACCATCAATAGATCGGAAGAGC1097840.33563885816506706No Hit
TCTGGGTGTCACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1045880.3197533055615393No Hit
TTGACGGCAACGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC958740.29311229220758617No Hit
ACTTTGACATTCAGAGCACTGGGCACCATCAATAGATCGGAAGAGCACAC887760.27141182023302113No Hit
ACGCCGTTAGGCGCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA884950.2705527285698973No Hit
AAATCATTACTCCGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACA833990.25497290253687627No Hit
TATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCGGAAG819490.25053986726452926No Hit
ATTACTCCGATCCCGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT803950.24578887635885524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACGTGGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG699400.2138251634123806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGCTACCCAGCGTTTACTGTAGGCACCATCAATAGATCGGAAGAGCA692960.21185628429831746No Hit
TCGACGGATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT671910.20542073998915159Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGAT653660.19984123007740448No Hit
AAAACTCAGCGGGTAATCCCGCCTGACCTGTAGGCACCATCAATAGATCG639880.19562831793582225No Hit
AGAAAAGATAACTCTTCCCGAGGCACCATCAATAGATCGGAAGAGCACAC628610.19218277948621182No Hit
AAATGTATCCAGAGCGTAGGCACCATCAATAGATCGGAAGAGCACACGTC626800.1916294143935947No Hit
AAATTTCTTCAAAGTAACAGCGCTGTAGGCACCATCAATAGATCGGAAGA623780.1907061201506645No Hit
AAAATACGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG590900.18065383051240447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTGGCTACCCAGCGTTTACCGTAGGCACCATCAATAGATCGGAAGAGC576610.17628499781986384No Hit
ACGTGGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA572880.17514463771187386Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAGTAACAGCGCCGTAGGCACCATCAATAGATCGGAAGAGCACACGTC570150.17431000417439058No Hit
TAAGGCTTTTGCTTGCTAAGTAAATGCTGTAGGCACCATCAATAGATCGG561680.17172050012219892No Hit
TATACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGA559760.17113350510682607No Hit
TTTGAAAAGAGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC557490.17043950579177589No Hit
TTTTGAAAAGAGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT550630.16834222151809997No Hit
ACTTTGACATTCAGAGCACTGTAGGCACCATCAATAGATCGGAAGAGCAC550470.1682933052668189No Hit
TATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT547220.16729969391267213No Hit
AAAGGCTTTTGCTTGCTAAGTAAATGCTGTAGGCACCATCAATAGATCGG508880.15557813719944558No Hit
CACATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC491760.15034409831237097No Hit
TTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG483240.14773930793165396No Hit
TCTGCCGAAGCTGTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC476740.14575208522336047No Hit
TACGAGGGACTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT468320.14317786749969413No Hit
AAAGTTGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC464180.14191215949779643No Hit
TTGCCGAAGCTGTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG461420.14106835416319796No Hit
TTTGGGAATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG442310.13522591940081505Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA432420.13220228361850386No Hit
ACCACCTGTGTCCGCGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCA431670.13197298869062385No Hit
ACGAGGGACTGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT427950.13083568584833896No Hit
AAATCATTACTCCGATCCCGTAGGCACCATCAATAGATCGGAAGAGCACA426340.1303434660698232No Hit
GTGACGGCAACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT425010.1299368497310493No Hit
AGTTTGTTTGATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC417440.12762249959231362No Hit
TTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCGGAA417080.1275124380269312No Hit
TACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATC405100.12384983371226105No Hit
CTGACGGCAACGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT402290.12299074204913724No Hit
AAAAAAGCCAGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC401310.12269113001004067No Hit
AAAAACTCAGCGGGTAATCCCGCCTGACCTGTAGGCACCATCAATAGATC384260.11747849198290156No Hit
ATTACTCCGATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC383720.11731339963482795No Hit
TCTGCCGAAGCTGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG374060.11436008096373332No Hit
TTGCCGAAGCTGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT369860.11307602936760519No Hit
TGTGCTTGCTAAGTACATGCTGTAGGCACCATCAATAGATCGGAAGAGCA368950.1127978181884441No Hit
AAAGTTCCCTGGAAAGGGGCGCTGTAGGCACCATCAATAGATCGGAAGAG366100.11192649746250002No Hit
ATCCTCTGTATTACCGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCA360090.11008908077375483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)