FastQCFastQC Report
Sat 2 Sept 2023
SRR12343670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39292224
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT5061941.2882803477858622No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG4003531.0189115281435839No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC3759710.9568585377096496No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3637680.9258015020987359Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG2621460.6671701759615338No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG2464370.6271902552525406No Hit
TCGGGGCTCAAACCACACACCGAAGCTACGGGCTGTAGGCACCATCAATA2407280.612660662832422No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC2332630.593661992764777No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG2094410.5330342207150198No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC1812170.46120321415250004No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA1781540.45340777859761766No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA1600150.40724342811442793No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG1580860.40233405978750403No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGA1552450.3951036215206347No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT1339770.3409758633158561No Hit
AGTAGGACGGAGCACGAGAAACTTTGTCTGACTGTAGGCACCATCAATAG1255410.3195059663713614No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG1225070.3117843367685168No Hit
CATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCA1184040.30134206707159156No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA1139530.2900141259502134No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT1078060.2743698091510422No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAATA1012910.2577889202708404No Hit
TCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGATC981370.24976188672852928No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA967540.24624210632617793No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG958890.24404065292919028No Hit
GGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT933840.23766534569282716No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT929730.2366193372001544No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGA923210.23495997579571978No Hit
TCGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC811330.2064861485061268No Hit
CTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAGA802510.20424142955104804No Hit
TCGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGCTGTAGGCACCATCAAT794230.20213414236872926No Hit
TCTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAATA773370.19682520388766997No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG749920.19085710190392888No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGA725820.18472357278631008No Hit
AGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCG710770.18089329837883444No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTCTGTAGGCACCATCAAT692030.17612390685749932No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA687800.17504735797087995No Hit
ACGAGCGCAACCCTTGTCCTTAGTTACCAGCTGTAGGCACCATCAATAGA671430.17088113922999115No Hit
GTCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGAT667890.169980197608565No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT658740.16765149257013295No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA627030.1595811934697308No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA617240.15708960633025No Hit
TGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT615490.15664422558519467No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCA613930.15624720046388824No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA579580.14750501269665978No Hit
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT576770.1467898584717424No Hit
GGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAATA569100.14483781829198572No Hit
GTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA563480.14340750984215095No Hit
CAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGATC562630.14319118205169554No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCAA555400.1413511233164099No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG543790.13839634020207153No Hit
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGTAGGCACCATCAATAG527720.13430647244604937No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA514490.13093939401343127No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATAGATCG505500.1286514095002614No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAAT502170.1278039135682419No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATA495960.12622344818150277No Hit
GGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAGA485170.1234773577591332No Hit
AAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAAG484440.12329157036262443No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG461550.11746599021730102No Hit
CCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG444860.11321833042588782No Hit
GGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGAT433100.11022537181911618No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA410950.10458812410313043No Hit
TAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGAT410780.10454485854503935No Hit
TCGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAG403330.10264880908751818No Hit
CGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATCA396180.10082911061486365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)