FastQCFastQC Report
Sat 2 Sept 2023
SRR12343674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9852849
Total Bases709.4 Mbp
Sequences flagged as poor quality0
Sequence length72
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAAT2599652.6384754297970057No Hit
GTTCGGATTGTTCTCTGCAACTCGGGAACATGAAGTCTGTAGGCACCATC1713391.7389792536148683No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCAT1425561.446850550536195No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA1203081.22104784108637No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA966540.9809751473913788No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACC953120.9673547214617823No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTCTGTAGGCACCATC882540.8957206184728904No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA806200.8182404906438737No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG784330.7960438650790244No Hit
TTCGGATTGTTCTCTGCAACTCGGGAACATGAAGTCTGTAGGCACCATCA736490.747489380990209No Hit
ACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGCTGTAGGCACCATC669790.6797932252894568No Hit
TCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATC665570.6755102001461709No Hit
TGTTCGGATTGTTCTCTGCAACTCGGGAACATGAAGTCTGTAGGCACCAT626070.6354202728571198No Hit
GCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATC586790.5955536312390457No Hit
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT585220.5939601834961644No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT561050.5694292077347374No Hit
ACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATC553970.5622434688687505No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAAT429290.4357013895168798No Hit
CGAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATCA414450.4206397560746135No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG412220.41837645131880136No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC406720.4127943095443764No Hit
ATGGCTCATTAAATCAGTTATAGTTTGTTTGATGCTGTAGGCACCATCAA371070.3766118814974227No Hit
GTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA370120.37564769337274934No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA353030.35830245647730924No Hit
TCTACCGTTGGTGTTAAAGGGAGAACTGCGAGGAGCTGTAGGCACCATCA336790.34181991421973484No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGCTGTAGGCACCA325690.33055413718407745No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA316280.3210036000754706No Hit
AAGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATACTGTAGGCACCAT312150.31681191907031153No Hit
AAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCAA309100.31371636772267597No Hit
TAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGAT304830.3093825958359861No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACTGTAGGCACCATCAAT304610.30915931016500914No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAATA290210.2945442480646968No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT278280.2824360750885353No Hit
CCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATC265090.26904908417859646No Hit
GGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACC244630.24828351677773608No Hit
TAAGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATACTGTAGGCACCA238720.24228525170739956No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA232290.23575922050566286No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGCTGTAGGCACCATCAATA230180.2336177079340199No Hit
AAAGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATACTGTAGGCACCA227430.23082663704680748No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGACTGTAGGCACCATCA218810.22207789848398166No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATA209850.21298408206600955No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG209420.21254766007273632No Hit
TAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTGTAGGCACCATCA203600.20664073914052677No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG199100.20207353223417918No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC198550.2015153180567367No Hit
TCTAAGTCAGAATCCGGGCTAGAAGCGACGCATCTGTAGGCACCATCAAT198510.20147472066201358No Hit
AGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCA194440.19734393574893921No Hit
AGAGTAGGGCGGGACACGTGGTATCCTGTCTGAACTGTAGGCACCATCAA190920.19377136501330733No Hit
AAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACC188000.19080775519852178No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCCTGTAGGCACCATCA183820.1865653274499589No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAAT183420.186159353502728No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGCTGTAGGCAC180470.18316529564190012No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGCTGTAGGCACCATCAA177860.1805163156362185No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGCTGTAGGCACC176550.17918675095903733No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTACTGTAGGCACCATCAATA171810.1743759596843512No Hit
CGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATCA170540.1730869924018931No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCAA170150.17269116780334298No Hit
AGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATACTGTAGGCACCATC169980.17251862887576985No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATA168780.17130070703407715No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG167930.1704380123962115No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA167360.16985949952140747No Hit
AAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCAC165680.1681544089430377No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG162560.1649878121546367No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCATC162500.16492691606255205No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAAT158950.16132389728087784No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAACTGTAGGCACCATCAAT154380.15668564493376483No Hit
ATCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCA152870.1551530932829682No Hit
TAATACCGCATAACGTCGCAAGACCAAAGAGGGGGCTGTAGGCACCATCA152570.15484861282254503No Hit
TCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCA148580.15079902269891685No Hit
GCCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAA144240.14639420537146158No Hit
TCACCAGCTAAGGCCCCTAAATGACCGCTCAGTGACTGTAGGCACCATCA143900.14604912751631532No Hit
ACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAGA138110.14017265463014808No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT132720.13470215569121174No Hit
AAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAATAGA127600.12950568916665625No Hit
ATCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTCTGTAGGCACCAT126930.1288256828050445No Hit
AGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATCTGTAGGCACCATCA126270.12815582579211354No Hit
GAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCTGTAGGCACCATCAA124400.1262578975888091No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTTCTGTAGGCACCAT122230.12405548892508148No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT120720.12252293727428483No Hit
CGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCAC119170.1209497882287651No Hit
TCGGATTGTTCTCTGCAACTCGGGAACATGAAGTCTGTAGGCACCATCAA118820.12059456102493807No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAAT118080.1198435092225609No Hit
TGTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAG117140.11888947044656831No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCTGTAGGCACCATCAA116850.11859513933482589No Hit
CAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAAT115450.11717423051951777No Hit
CAGTCGAAGATACCAGCTGGCTGCAACTGTTTATTACTGTAGGCACCATC115010.11672765917756377No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAAGCTGTAGGCACCATCAA110450.11209955617913153No Hit
AAAACCCTCGTCTTGTGTTGCTCAGACATGCGCCTACTGTAGGCACCATC109930.11157179004773138No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA108630.11025237471923095No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCA107780.1093896800813653No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAA106390.10797892061473793No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT104750.10631442743109125No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGCTGTAGGCACCATCAATAG104590.1061520378521989No Hit
TTAGGATATCTGGCTTTCACCCAGGAGACCCGGGCTGTAGGCACCATCAA104250.10580695999705261No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCATCAATAGAT103670.10521829777356782No Hit
TCTACCGTTGGTGTTAAAGGGAGAACTGCGAGGACTGTAGGCACCATCAA101650.10316812934005179No Hit
ATGGCGCTTAAGCGCGCGACCTATACCCGGCCTGTAGGCACCATCAATAG98790.10026541561735088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)