FastQCFastQC Report
Sat 2 Sept 2023
SRR12343676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32577272
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG2695710.8274818100177326No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG2549320.7825455734906225No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2253700.6918013270110523Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC1820990.5589755950099198No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT1604420.4924967320775049No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA1517700.4658769463569571No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG1385060.4251614438434256No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGA1182270.3629125237988006No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA1173010.36007005129220154No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG1167730.35844928943098736No Hit
AAACTCGACGGATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1070150.32849589124589684No Hit
AAAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATCGGAAG1058720.32498731017133664No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC988580.3034569622649803No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT883830.27130264314335467No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG839730.25776559805253185No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG734530.22547314581773453No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT724100.22227152721688911No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAATA719980.22100684182518415No Hit
AAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAAG699810.21481540873035657No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA655370.2011739963984707No Hit
AGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAGATC652450.20027766597522345No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA648300.19900377170930703No Hit
GGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT644560.19785573205761364No Hit
ACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGG634650.19481373394309995No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA631470.1938375932766869No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA597310.1833517551745892No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA589780.18104032774751674No Hit
TGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT585580.17975108535791456No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG583500.17911260341258778No Hit
GGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAATA581580.17852323546305535No Hit
ATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGATCGGA577170.17716953095397306No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAAT545660.1674971434072196No Hit
AAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGATCGGAA502100.15412585805220277No Hit
AGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCG501940.15407674405640842No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA491110.15075234046607708No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAGA489550.150273479007082No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG474240.14557388353450834No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA463620.14231394206365716No Hit
ATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAGATCGGA439780.13499595669029624No Hit
AAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAGA431200.1323622186658232No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC415540.1275551863274494No Hit
TAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAG398020.12217720378796604No Hit
CCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG396750.12178736144634825No Hit
CTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAGA385180.11823580562546797No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTCTGTAGGCACCATCAAT377360.1158353590810182No Hit
ATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGAA377200.11578624508522384No Hit
TGCATTTATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATA374660.11500656040198823No Hit
AGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGATCGGAAGA366250.11242500599804675No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA366120.1123851008764638No Hit
GGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAGA357830.1098403819693681No Hit
TCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGATC354540.10883047543084638No Hit
AGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAAT349340.1072342705675294No Hit
TCTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAATA349070.10715139069962643No Hit
AAAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCA347930.10680145347959154No Hit
TAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAA346130.10624892102690489No Hit
GGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGAT341880.10494433051361697No Hit
CTCTAGGGATAACAGGCTGATCTTCCCCAAGCTGTAGGCACCATCAATAG337900.10372261986823206No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT334700.10274033995234469No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG334350.1026329030865445No Hit
GAGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATA328420.10081261561741572No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA326810.1003184060347349No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACCTGTAGGCACCATCAATAG325910.10004213980839156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)