FastQCFastQC Report
Sat 2 Sept 2023
SRR12343687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40787403
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT4017100.984887417323432No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG3483070.8539572867632685No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC3098580.7596904367752956No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC2636280.6463466183419425No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA2611650.6403079892093154No Hit
TGTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAG2594660.6361424874243649No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG2584350.6336147461999481No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG2522970.6185659822470188No Hit
GTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA2163660.5304726069468066No Hit
TAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGAT2049330.5024418936405438No Hit
TTGAAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAAT1839470.4509897332762275No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1726240.42322871108023224Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAAT1709680.41916863400202264No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG1690460.4144563947844387No Hit
TGAAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATA1665020.4082191749251601No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT1592170.39035826821335007No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG1579940.38735979341464816No Hit
ACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAGA1563680.38337326845742054No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA1550920.38024485157831694No Hit
TAGAGTAGGGCGGGACACGTGGTATCCTGTCTGAACTGTAGGCACCATCA1497140.3670594080235999No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATA1447430.35487182157687264No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC1373700.33679516197684856No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCA1263760.30984076137429No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG1173280.28765744168610097No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG1172880.2875593721914582No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA1146440.28107697859557274No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG1119270.27441560817196425No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG1117220.2739130020119202No Hit
AGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT1061730.26030831136760535No Hit
TTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA1035580.25389701815533583No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCAT981740.2406968641764223No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT976020.23929447040303103No Hit
TCGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAG926570.22717062912782165No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA909460.2229757064944782No Hit
TCAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGAT845360.20726006997797827No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG836080.20498485770226657No Hit
TCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGATC783450.1920813639446473No Hit
TAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCA774120.18979389298210528No Hit
TATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAG774030.18977182734581066No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA753560.18475312095746818No Hit
AACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATA751530.18425541827215625No Hit
TGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAAT745290.1827255341557294No Hit
TTAGAGTAGGGCGGGACACGTGGTATCCTGTCTGAACTGTAGGCACCATC736240.18050671183943728No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA726610.1781456887559132No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCAA715600.17544632591587161No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA698640.1712881793430192No Hit
TTTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG695910.1706188550420825No Hit
TTGAGCTAACCGATACTAATGAACCGTGCTGTAGGCACCATCAATAGATC680890.16693634551824738No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT673850.16521032241253508No Hit
TCGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC667300.1636044344377601No Hit
AAAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAAT662350.16239082444155614No Hit
TATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATA649590.15926240756245255No Hit
TAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAACTGTAGGCACCATCAA644220.15794582459687367No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA635580.1558275235125904No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAACTGTAGGCACCATCAAT612270.1501125237122844No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA608610.14921518783630328No Hit
TGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAGA608080.14908524575590165No Hit
TAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCTGTAGGCACCATC605480.1484477940407238No Hit
AGTAGGGCGGGACACGTGGTATCCTGTCTGACTGTAGGCACCATCAATAG599140.1468933925506363No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT592150.1451796281317543No Hit
TCGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG591730.14507665516237941No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT582630.14284557415925697No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCTGTAGGCACCATCA581570.1425856899984537No Hit
CCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC581090.1424680066048824No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTTCTGTAGGCACCATCAATAG575370.14106561283149113No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA557980.13680204155189776No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCATCAATAGAT542550.13301901079605388No Hit
AAACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATA528560.12958903022092383No Hit
TTGGCGCTTAAGCGCGCGACCTATACCCGGCCTGTAGGCACCATCAATAG525720.12889273680896035No Hit
AAACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAAT511600.12543088364807142No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAATA496650.12176553628579882No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACTGTAGGCACCATCAAT491130.12041217725972894No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT487500.11952219659584604No Hit
AACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAG486560.11929173328343558No Hit
TACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGA478410.11729356733008964No Hit
CAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGATC476310.11677870248321522No Hit
TAGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAA472680.11588872181933231No Hit
AGTGATAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATA465200.11405482226951297No Hit
AAAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAA463560.11365273734147772No Hit
AACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATA454960.11154424320665868No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATA452440.11092640539040939No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG449220.11013694595853529No Hit
ATGTACACGGAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA443380.10870513133675119No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT440250.10793773754117172No Hit
CCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACTGTAGGCACCATCAAT433070.10617739011233443No Hit
TAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACC428820.10513540173175528No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCAT420820.10317401183890036No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA408010.10003333627296644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)