FastQCFastQC Report
Sat 2 Sept 2023
SRR12343688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31152596
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG4613721.481006590911396No Hit
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT3988851.2804229862577101No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAAT3722331.1948699235209805No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3689761.1844149360778795Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGA2626490.8431046966358758No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG2445980.7851608899624287No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCAT2202090.7068720693453605No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG2045490.6566033854770883No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC2026490.6505043753014997No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT1916300.6151333262884415No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAATA1782750.5722637047647651No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG1627630.5224701016891177No Hit
ACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAGA1567890.5032935296949249No Hit
TGAAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATA1531410.4915834301577948No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG1428440.4585300050114604No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATA1426400.4578751639189235No Hit
AGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT1329710.4268376221358888No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA1247660.4004995281934129No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACC1191040.3823244778701589No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTGTAGGCACCATCAA1183630.37994586390167934No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG1112560.3571323558396225No Hit
GTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA1111710.35685950538439876No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTCTGTAGGCACCATCAAT1028980.3303031310777439No Hit
GTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA1023780.328633928292846No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATA977180.3136753033358761No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA945930.3036440365997107No Hit
GCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCATC932850.29944534959462127No Hit
GAAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAG854530.274304587649774No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA765310.24566491986735228No Hit
AACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAG763790.24517699905330523No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATA733960.23560155307763117No Hit
GTTCGGATTGTTCTCTGCAACTCGGGAACATGAAGTCTGTAGGCACCATC712440.2286936215524382No Hit
TTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGACTGTAGGCACCA705060.22632463760002536No Hit
GGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAGA702140.22538731603619805No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCAA686230.22028019751548153No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAA670860.21534641928396595No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCATC664910.21343646609740005No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACCTGTAGGCACCATCAATAG643940.20670508486676362No Hit
AAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAATAGA601170.19297589196097814No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAAT588690.18896980527722312No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCCTGTAGGCACCATCAATAGA579200.18592351019478442No Hit
GTCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGAT578130.1855800396217381No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACCTCTGTAGGCACCATC577670.18543237937538173No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC572130.18365403640839434No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG563410.18085491173833473No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG559790.17969288979961734No Hit
CTCTAGGGATAACAGGCTGATCTTCCCCAAGCTGTAGGCACCATCAATAG548710.17613620386564252No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA543250.17438354094149972No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA542760.17422625067907663No Hit
TCTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAATA523580.16806946040708776No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCCTGTAGGCACCATCAATAGAT515360.1654308360048068No Hit
AAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGATCGGAA513420.16480809496582566No Hit
TCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGATC494280.15866414471525905No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCTGTAGGCACCATCA490950.15759521293185325No Hit
AAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGA480350.15419260725494593No Hit
GAAGGGGTAGAGAAAATGCCCCGAGCCAATGTTCCTGTAGGCACCATCAA457790.1469508351727734No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAAT451210.1448386516488064No Hit
ATGGCGCTTAAGCGCGCGACCTATACCCGGCCTGTAGGCACCATCAATAG445980.14315981884784176No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT440940.141541976148633No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTTCTGTAGGCACCATCAATAG439560.14109899540956394No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAG437330.14038316421527117No Hit
CTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAGA435170.13968980305846743No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAACTGTAGGCACCATCAAT428100.13742032927207737No Hit
GGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGAT426810.13700623858120844No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC425530.13659535789569513No Hit
AAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAATAG421430.1352792556999102No Hit
CATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCA420570.13500319523933094No Hit
TCAGGGCTCAAGTGATTCACCGAAGCGACGAGACTGTAGGCACCATCAAT417640.13406266367014807No Hit
TAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGAT416440.1336774630274793No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA415620.13341424258832235No Hit
GTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAGAT412880.13253470112089535No Hit
TGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGCTGTAGGCACCA410140.13165515965346836No Hit
TAGTAATCGCGGATCAGCATGCCGCGGTGAATCTGTAGGCACCATCAATA406180.13038399753266147No Hit
ACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATC404100.12971631641870232No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT386650.12411485707322753No Hit
GGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAATAG381720.12253232443293009No Hit
CGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAGA374270.12014087044302825No Hit
AGGACGCACCTCTGGTGTACCAGTTATCGTGCTGTAGGCACCATCAATAG370690.11899168852573312No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAATA370240.11884723828473236No Hit
GATCGTACGCTAAGGTCCCTAAGCAATCACTCTGTAGGCACCATCAATAG368460.11827585733144036No Hit
AGTAGGGCGGGACACGTGGTATCCTGTCTGACTGTAGGCACCATCAATAG365630.11736742581581322No Hit
ACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGCTGTAGGCACCATC365360.11728075567121277No Hit
GTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGATC357120.11463571125822065No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC355480.1141092703799067No Hit
AAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAATAG353670.11352825941054799No Hit
TGTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAG353660.11352504940519244No Hit
TGTTCGGATTGTTCTCTGCAACTCGGGAACATGAAGTCTGTAGGCACCAT351670.11288625833943342No Hit
ACTCATCTTCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATAG344790.11067777465479922No Hit
CAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGATC339660.10903104190739031No Hit
ATCGTACGCTAAGGTCCCTAAGCAATCACTCTGTAGGCACCATCAATAGA333700.10711787871546885No Hit
GGGTCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAG331910.1065432877568213No Hit
ATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAGATCGGAA330750.10617092713557483No Hit
TCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAAT322400.10349057266367144No Hit
AGTAATCGCGGATCAGCATGCCGCGGTGAATCTGTAGGCACCATCAATAG319390.10252436105164398No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCA317380.10187914997517383No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT313720.10070428801503412No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG312950.100457117602655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)