FastQCFastQC Report
Sat 2 Sept 2023
SRR12343689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25163342
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT6235782.4781207520050397No Hit
AGTAGGGCGGGACACGTGGTATCCTGTCTGACTGTAGGCACCATCAATAG5202982.067682424695416No Hit
TTGGTTGATGCCATGCTTCCAAGAAAAGCTCCTGTAGGCACCATCAATAG4038191.604790810378049No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA2864941.1385371625120384No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT2565681.01961019327242No Hit
TCGGGGCTCAAACCATGCACCGAAGCTACGGGCTGTAGGCACCATCAATA1852450.7361701001401165No Hit
AAAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCA1669720.6635525599103649No Hit
AAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGATCGGAA1513420.6014383939939297No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG1504940.5980684123754308No Hit
TACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGA1475090.5862059181169179No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA1409530.5601521451323914No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG1331340.5290791660344639No Hit
TAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGAT1281460.5092566798162184No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT1223620.4862708617957026No Hit
TTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA1121350.4456284065924153No Hit
TGTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAG1079760.42910039532904654No Hit
AGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT1029450.40910702560891954No Hit
ACGAGCGCAACCCTTGTCCTTAGTTACCAGCTGTAGGCACCATCAATAGA997290.3963265292821597No Hit
ACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAGA951270.37803802054591956No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAAT860110.34181071814705694No Hit
TCGGGGCTGAAGCCATGCACCGAAGCTGAGGACTGTAGGCACCATCAATA845740.3361000299562753No Hit
TCAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGAT831510.33044497825447827No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG797200.3168100644183114No Hit
AAAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGATCGGA773950.30757043321193184No Hit
TTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC768170.3052734410238513No Hit
TAGGGCGGGACACGTGGTATCCTGTCTGACTGTAGGCACCATCAATAGAT749080.2976870083473014No Hit
ACTCCGCAAGTAGTGGCGAGCGAACGCGGACCTGTAGGCACCATCAATAG710200.2822359605492784No Hit
ACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGATCGGAAGAGCAC700820.2785083157873068No Hit
TCGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAG690550.2744269819167899No Hit
TTGATAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATAG662040.26309700833855854No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC645050.2563451229967784No Hit
TTCAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGA631520.25096825373990467No Hit
AGGGCGGGACACGTGGTATCCTGTCTGACTGTAGGCACCATCAATAGATC602330.23936804578660498No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC600680.23871233002357156No Hit
ACTCCGCAAGTAGTGGCGAGCGAACGCGGACCCTGTAGGCACCATCAATA593770.23596627188868635No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATAGATCG551560.21919187045981414No Hit
TAGTAGGGCGGGACACGTGGTATCCTGTCTGACTGTAGGCACCATCAATA538920.21416869031148567No Hit
TCGAGCGCAACCCTTGTCCTTAGTTACCAGCTGTAGGCACCATCAATAGA533530.2120266854855766No Hit
AGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGATC514980.20465485069511036No Hit
TTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCAAT509380.20242939113572436No Hit
TACGAGCGCAACCCTTGTCCTTAGTTACCAGCTGTAGGCACCATCAATAG485790.19305464274181067No Hit
TTGAAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAAT472690.18784865698681835No Hit
ACGAGCGCAACCCTTGTCCTTAGTTACCACTGTAGGCACCATCAATAGAT467450.18576626268482144No Hit
TACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAG456590.1814504607535835No Hit
AAGTGGTTGATGCCATGCTTCCAAGAAAAGCTGTAGGCACCATCAATAGA455360.1809616544575041No Hit
AAAGTGGTTGATGCCATGCTTCCAAGAAAAGCTGTAGGCACCATCAATAG414750.1648230986170279No Hit
TTCGATGGCCCGAGGTAGAGCACTCAATGGGCTACTGTAGGCACCATCAA409370.16268506782604633No Hit
CTGGTTGATGCCATGCTTCCAAGAAAAGCTCCTGTAGGCACCATCAATAG402150.15981581460840932No Hit
GTGGTTGATGCCATGCTTCCAAGAAAAGCTCCTGTAGGCACCATCAATAG387380.15394616502052869No Hit
AAGAAGCGCATACGGTGGATGCCTTGGCACTGTAGGCACCATCAATAGAT380740.15130740582868524No Hit
TTGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGA362570.14408658436546307No Hit
AAAAGGGCCGTCGAAGACTACGACGTTGATCTGTAGGCACCATCAATAGA358880.14262016547722478No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCACTGTAGGCACCATCAATAGAT355920.14144385113869215No Hit
AGTAGGACGGAGCACGAGAAACTTTGTCTGACTGTAGGCACCATCAATAG349050.13871368914351678No Hit
TGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAGA328310.13047154070393352No Hit
ATCCTTGTGTCCCTGGTTCGATTCCTGTAGGCACCATCAATAGATCGGAA328130.13040000807523897No Hit
GGGGAAACTTGCCTCAAGATGAGATCTCACCTGTAGGCACCATCAATAGA326650.12981185090597266No Hit
ACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAATAGATCGGAA320590.12740358573992278No Hit
ATCCGTGTGTCGACAGTTCGATTCTGTCCTGTAGGCACCATCAATAGATC315730.1254722047651699No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCACTGTAGGCACCATCAATA311920.12395809745780191No Hit
AGTAGGGCGGGGCACGTGAAACCCTGTCTGACTGTAGGCACCATCAATAG311100.12363222659374895No Hit
AAACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAAT306350.1217445600031983No Hit
TAAGTGGTTGATGCCATGCTTCCAAGAAAAGCTGTAGGCACCATCAATAG299700.11910182677642739No Hit
TGGTTGATGCCATGCTTCCAAGAAAAGCTCCTGTAGGCACCATCAATAGA298200.11850572153730614No Hit
TTGAGCTAACCGATACTAATGAACCGTGCTGTAGGCACCATCAATAGATC298120.11847392925788633No Hit
GTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA293900.11679688651849186No Hit
TCGGGAAACTTGCCTCAAGATGAGATCTCACCTGTAGGCACCATCAATAG290560.1154695588527152No Hit
GCGGGGCTCAAACCATGCACCGAAGCTACGGGCTGTAGGCACCATCAATA282710.11234994143464727No Hit
TTGTCGATGGCCCGAGGTAGAGCACTCAATGGGCTCTGTAGGCACCATCA280910.11163461514770176No Hit
GTGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGA277540.11029536537714268No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA277060.11010461170062387No Hit
TAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGA276280.10979463697628081No Hit
ACGGGGCTCAAACCATGCACCGAAGCTACGGGCTGTAGGCACCATCAATA266480.10590008274735527No Hit
AAAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATAGATC266060.10573317328040131No Hit
AAAGCGTACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAATAG261020.10373025967695389No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)