FastQCFastQC Report
Sat 2 Sept 2023
SRR12343690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36944312
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3977611.0766501755398774Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG3100200.8391548880379746No Hit
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT3040710.8230522739197308No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC2943030.796612479885943No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC2673880.7237595871321139No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA2449200.6629437300118081No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG2140600.5794126034881906No Hit
TGTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAG2098650.5680576755631557No Hit
TTGAAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAAT1756140.47534786951777586No Hit
GTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA1671590.45246207318734205No Hit
TAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGAT1615230.4372066801514669No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCCTGTAGGCACCATCAATAG1566930.42413294907210614No Hit
TGAAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATA1501110.4063169453527785No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG1455680.3940200591636407No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG1334960.36134385179510176No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT1326610.35908369331657874No Hit
ACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAGA1271600.34419371512453667No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATA1268120.34325175686043363No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA1257430.3403582126525999No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC1201520.3252246245646691No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCA1176960.31857678118352833No Hit
TAGAGTAGGGCGGGACACGTGGTATCCTGTCTGAACTGTAGGCACCATCA1127800.3052702673147628No Hit
ACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG1030720.278992879878234No Hit
ACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATAG1016540.27515467062967636No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATA954040.25823731674851597No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG944400.25562798408588583No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG943300.25533023865757737No Hit
AGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT925920.2506258608903043No Hit
TGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAAT848770.2297430792593999No Hit
TCGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAG830820.22488441522473065No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAAT801260.21688318353309705No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG767820.20783172251252102No Hit
TATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAG757320.20498960706048608No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAATA738240.19982507726764542No Hit
TCAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGAT708220.19169933385144647No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA699430.18932007720160007No Hit
TCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGATC682520.1847429179355133No Hit
AACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAATA667220.18060154970540526No Hit
TTTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG627790.1699287294888588No Hit
TAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCA621820.16831278384613035No Hit
TCGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC616180.16678616183189446No Hit
AAAAAGCCCGATCCAGCAATATCGCGTGAGTGACTGTAGGCACCATCAAT601850.162907350934022No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT580640.1571662777209114No Hit
TATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATA579260.15679274254721537No Hit
TTGAGCTAACCGATACTAATGAACCGTGCTGTAGGCACCATCAATAGATC566730.15340115144112035No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATCTGTAGGCACCATCAA564700.15285167578706027No Hit
TAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCTGTAGGCACCATC540100.14619300529943555No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA531360.14382728253269406No Hit
TTAGAGTAGGGCGGGACACGTGGTATCCTGTCTGAACTGTAGGCACCATC531050.1437433724574435No Hit
AGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACCATCAATAGAT527070.14266607536229123No Hit
CCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC523990.14183238816302762No Hit
TGCCATTTAAAGTGGTACGCGAGCTGGGTTCTGTAGGCACCATCAATAGA516320.13975629049473162No Hit
AAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAAT509120.13780741132762195No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCTGTAGGCACCATCA501550.1357583814255358No Hit
TGTCGATGGCCCGAGGTAGAGCACTCAATGGGCCTGTAGGCACCATCAAT496160.13429942882682455No Hit
TTGGGTTGGGCCAACCTATGGTGTACCGGTTCTGTAGGCACCATCAATAG484200.1310621239881257No Hit
AAACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATA482210.13052347544054954No Hit
TAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAACTGTAGGCACCATCAA480610.13009039118119184No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAACTGTAGGCACCATCAAT457410.12381066942050512No Hit
AAACGAGCGCAACCCTCGTGTTTAGTTGCCACCCTGTAGGCACCATCAAT455650.12333427673521165No Hit
TAGGAAAGGCTTACGGTGGATACCTAGGCACCCACTGTAGGCACCATCAA441580.1195258420294848No Hit
ACACGGTGGATGCCCTGGCAGTCACTGTAGGCACCATCAATAGATCGGAA439880.11906569000391726No Hit
TAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGCTGTAGGCACC430740.11659169617233636No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT424250.11483499814531667No Hit
TAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAA417280.11294837484048965No Hit
AAATGCATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAATA412720.1117140847013202No Hit
AAAAAGCCCGATCCAGCAATATCGCGTGAGTGAACTGTAGGCACCATCAA411940.1115029561248833No Hit
AGTGATAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATA408220.11049603522187663No Hit
AGTAGGACGGAGCACGAGAAACTTTGTCTGACTGTAGGCACCATCAATAG402350.10890715734535807No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCAT398700.1079191838786983No Hit
CAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGATC391510.1059730114882096No Hit
AACGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATA390140.10560218309113456No Hit
AACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAG388700.10521240725771264No Hit
AAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAAG375940.10175856028933493No Hit
TACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGA373350.10105750514449964No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG370130.10018592307254226No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)