FastQCFastQC Report
Sat 2 Sept 2023
SRR12343695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18927660
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA14518197.670356504713208No Hit
AAAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCA8108444.283910425271798No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT7282233.8474011050494354No Hit
AAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAG1459900.7713050635947603No Hit
AAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA1405120.7423632926626957No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAG945410.4994859375115571No Hit
ATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGAA945110.4993274393136816No Hit
TTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACTGTAGGCACCATCAA711690.37600527482002527No Hit
AAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCTGTAGGCACCATCA686020.36244311235514587No Hit
ATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGATCGGAAG653690.34536228989743056No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640920.33861554994119714Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCGTGTGTCGACAGTTCGATTCTGTCCTGTAGGCACCATCAATAGATC609940.3222479693739216No Hit
AAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAGATCGGAAG577900.3053203618408192No Hit
TAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATA550850.29102910766571255No Hit
TTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACCTGTAGGCACCATCA527720.2788088966095122No Hit
ATTAGCTGATGATTCATGATAACTCTGTAGGCACCATCAATAGATCGGAA505980.2673230605368017No Hit
TATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGA474990.25095019669626356No Hit
TAGGATACTCGGCTCTCACCCGAGAGACCCGGGTCTGTAGGCACCATCAA467960.24723605559271458No Hit
ACCTCAGCCTGCTAACTAGCTACGTGGAGGCCTGTAGGCACCATCAATAG452800.2392266133267398No Hit
ATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAGATCGGA446720.23601438318312987No Hit
AGGTCAGGCGGGATTACCCGCTGAGTTCTGTAGGCACCATCAATAGATCG397990.21026899257488776No Hit
TATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGATCGGAA393160.2077171715890924No Hit
AGGTCAGGCGGGATTACCCGCTGAGTCTGTAGGCACCATCAATAGATCGG382990.20234408268111326No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG374380.1977951844020867No Hit
AGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAATAGA358900.1896166773917114No Hit
TTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC343380.18141703728828604No Hit
ATCCTTGTGTCCCTGGTTCGATTCCTGTAGGCACCATCAATAGATCGGAA336990.17804102567353808No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA319230.1686579323593091No Hit
ACTCGGCTCTCACCCGAGAGACCCGGGTCTGTAGGCACCATCAATAGATC290390.15342097227021195No Hit
ATTTATTAGCTGATGATTCATGATAACTCTGTAGGCACCATCAATAGATC278070.1469119796107918No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGA265850.14045581968399687No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC260600.13768210122117577No Hit
ACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCACTGTAGGCACCATCAATA260420.13758700230245047No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGCTGTAGGCACCATCAAT252570.13343963279137516No Hit
TAAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAG252220.1332547182271871No Hit
TATTAGCTGATGATTCATGATAACTCTGTAGGCACCATCAATAGATCGGA234550.12391917437232071No Hit
TACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATA231700.12241344149250358No Hit
TATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGAT230980.1220330458176024No Hit
TAAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAGATCGGAAG224260.1184826861851914No Hit
TTAGGATACTCGGCTCTCACCCGAGAGACCCGGGTCTGTAGGCACCATCA217940.11514365748328108No Hit
ATTAGCTGATGATTCATGATAACTCCTGTAGGCACCATCAATAGATCGGA217270.11478967817469249No Hit
TAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA214850.11351112604516353No Hit
TGAATGCATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAG214020.11307261436437467No Hit
AAAACTCAGCGGGTAATCCCGCCTGACCTGTAGGCACCATCAATAGATCG204530.10805878803824666No Hit
AAAACAGAAAAGATAACTCTTCCTGAGGCACCATCAATAGATCGGAAGAG201860.10664815407715482No Hit
TATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAGATCGG200830.10610397693111562No Hit
TAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCTGTAGGCACCATCA192640.10177697612911475No Hit
TAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAA189880.10031879270866023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)