FastQCFastQC Report
Wed 20 Sept 2023
SRR12343696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195453612
Total Bases23.5 Gbp
Sequences flagged as poor quality0
Sequence length72-122
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA167149338.551867028172392Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCCGGCAACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG140321937.179295821864883No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC48074702.45964756077263No Hit
TCCCCACAGACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT24600881.25865568552399No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG23012311.1773796229460318No Hit
GTGTAGTGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA18467850.9448712567153785No Hit
ACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC18414370.942135057601289No Hit
AACCTGCATGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT16779400.8584850301973442No Hit
ATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC15265250.7810165206872719No Hit
TCCCCGGCAACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT13166800.6736534498016848No Hit
GTCTAGTGGTAGAATAGTACCCTGTAGGCACCATCAATAGATCGGAAGAG12841630.657016765696814No Hit
AAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAAG12450380.6369992282363142No Hit
TCCCCACAGACGGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG11746830.6010034749319445No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG11497240.5882336930156092No Hit
TGCGAGAGGCACGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC11240980.5751226536555385No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC10872060.5562475867675446No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGA10772480.5511527717379815No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC10539490.539232296203357No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA9504590.4862836712375518No Hit
AGGTGCGGCTGGATCACCTGTAGGCACCATCAATAGATCGGAAGAGCACA9461520.48408007931825786No Hit
ATCTCTCAGGCGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC9461140.4840606373649416No Hit
AACCTGCATGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC9065750.46383128493936454No Hit
TTCCCGGCTGGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT9061940.4636363537758514No Hit
TCCCTGTATAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT9051540.4631042582114062No Hit
ATGTACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGA8838540.4522065317472874No Hit
TTCCCGGCTGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC7902890.40433583800948125No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT7585780.3881115279670554No Hit
GTCTAGTGGTCAGGACATTGGACTGTAGGCACCATCAATAGATCGGAAGA7235820.3702065122234733No Hit
AACTCGACGGATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG6826060.349241946984331No Hit
TCCCCGGCAACGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC6542230.3347203427481299No Hit
TGCGAGAGGTACGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6352510.32501369173980776No Hit
GTCTAGCGGTTAGGACATTGGACTGTAGGCACCATCAATAGATCGGAAGA6051850.3096310136238362No Hit
TCCCCGGCAACGGCGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA5926610.3032233551150746No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC5342520.27333953797691907Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACCCCGCATCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5311770.2717662746493526No Hit
TTTGTCGCTAAGATTCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC5202180.26615931763901096No Hit
TACCCCGCATCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACG4995520.2555859648170636No Hit
TGCGAGAGGCACGGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA4886630.2500148219312519No Hit
TCCCCACAGACGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4750520.2430510212315749No Hit
AAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAGA4431250.22671619903345658No Hit
TCCACGCTCACCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4364940.22332357817976778No Hit
TCCCCCGCATCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4224740.21615052066676568No Hit
GTGTAGTGGTTATCACTTTTGCCTGTAGGCACCATCAATAGATCGGAAGA4196500.21470567655715672No Hit
GTCTAGTGGTAGAATAGTACCCCTGTAGGCACCATCAATAGATCGGAAGA4168270.21326134407789812No Hit
GTCTAGTGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC4125980.21109765932593766No Hit
ACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA3930200.20108096032525608No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3741530.19142803050372895Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTGCGGAAGGATCATTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC3693760.18898397231973385No Hit
TATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA3329290.1703365809376805No Hit
GTCTAGTGGTCAGGACATTGGACTCTGTAGGCACCATCAATAGATCGGAA3326200.1701784871593982No Hit
ACCCGGGCGAAGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3182460.16282431250234455No Hit
ACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA3031190.1550848801914185No Hit
ACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAA2982290.15258300777782505No Hit
GTGTAGTGGTTAGCACTCAGGACTGTAGGCACCATCAATAGATCGGAAGA2945990.15072578960577102No Hit
ATCCTGCCGTTCACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2753930.1408994170954487No Hit
AAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAGATCGGAAGA2681150.13717577140503293No Hit
CCCCTACCTGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2681130.1371747481443321No Hit
TTCTCGGAACGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2658800.13603227757182612No Hit
ACCCGGGCGAAGCCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2606580.1333605438818905No Hit
ATCCCAGCAGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2584670.1322395617841025No Hit
AGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCG2475150.12663618618621383No Hit
ATTACCCGCTGAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2467800.12626013787864918No Hit
CCGTGGAGACCTGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2392670.12241625905588277No Hit
ACACGGAGTCAAGACCCGCAACGCGACTGTAGGCACCATCAATAGATCGG2362450.12087011213688904No Hit
GCCCCACGGTGGGCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2320910.11874479966121065No Hit
GTCTAGTGGTAGAATAGTACTGTAGGCACCATCAATAGATCGGAAGAGCA2317670.11857903142767195No Hit
GTCCCGGCAACGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2313910.11838665841591098No Hit
ATCTCTCAGGCGACGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2271720.11622808996745479No Hit
TCCCTGTATAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2205960.11286360878303953No Hit
AAACTCGACGGATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2164000.11071680783264316No Hit
GTGTAGTGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG2097470.10731293111124496No Hit
TAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAA2089930.10692716182702217No Hit
AGGTGCGGCTGGATCACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA2067970.10580362157748203No Hit
GCGCAGCGGAAGCGTGGTGGGCTGTAGGCACCATCAATAGATCGGAAGAG2056110.1051968279818743No Hit
GTCTAGTGGTATGATTCGCGCCTGTAGGCACCATCAATAGATCGGAAGAG2041740.1044616151683091No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG2015720.10313035299649514No Hit
TCCACGCTCACCGCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1970910.10083773739622678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)