FastQCFastQC Report
Sat 2 Sept 2023
SRR12343700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26984239
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length72
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7331532.716967486094383Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGATCGGAA5881762.1797020104958307No Hit
AAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAAT3714461.3765294622538735No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATAGATCG2751341.0196100027130652No Hit
ACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCG2351040.8712641479346518No Hit
ATTCCCGTAGTAGCGGCGAGCGAAATGGGAGCTGTAGGCACCATCAATAG2120600.785866149495637No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAA1850970.6859448584041966No Hit
TCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAAT1598570.5924087761007453No Hit
AAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAAG1574690.583559165778216No Hit
ACGAAACTTGCCCCGAGATGAGTTCTCCCCTGTAGGCACCATCAATAGAT1561440.5786488920439816No Hit
ATTGGATGGATGCCCGGGCATTGACTGTAGGCACCATCAATAGATCGGAA1455420.539359290436169No Hit
ATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGATCGGAAGA1321190.489615438108149No Hit
ATACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGAT1264580.4686365251953186No Hit
TCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATC1200330.444826329917994No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATAG1082640.401211981557086No Hit
ATTGGATGGATGCCCGGGCATTGAGCTGTAGGCACCATCAATAGATCGGA1018110.37729802200462276No Hit
TGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT997500.36966023018103267No Hit
AGAAATCACATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAGA894170.3313675067879439No Hit
TCTGATGATTCATGATAACTCGACGGATCGCCTGTAGGCACCATCAATAG876580.3248488867890623No Hit
AGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAGATC875420.32441900622063125No Hit
AGTAGCATGGGGCACGTGGAATCCCGTGTGACTGTAGGCACCATCAATAG873220.32360371548739986No Hit
AAAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGATCGGA853940.3164588039707179No Hit
TGGTTGATGCCATGCTTCCAAGAAAAGCTCCTGTAGGCACCATCAATAGA720090.2668557745875287No Hit
AAAAAGCTCGTAGTTGAACCTTCTGTAGGCACCATCAATAGATCGGAAGA718430.26624060067063593No Hit
AAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGCTGTAGGCACCATCAA681920.2527104803659648No Hit
ATTGGATGGATGCCCGGGCATTGCTGTAGGCACCATCAATAGATCGGAAG667300.24729250285694548No Hit
ATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC665120.24648462385765263No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTGTAGGCACCATCAATAGATCGGA656550.24330869586501958No Hit
ACTGGGCAGAAATCACATTGCGTTAGCATCTGTAGGCACCATCAATAGAT655810.24303446170929632No Hit
TATACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAATAGA615790.2282035820984242No Hit
TCGGGGCTAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAG605260.22430130417982141No Hit
ATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTGTAGGCACCATCAAT598540.22181096157649657No Hit
TCCCCGGCAACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC571590.2118236500944125No Hit
AGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATAGAT549260.20354844915211429No Hit
AGAGCACTGGGCAGAAATCACATTGCGTTAGCATCTGTAGGCACCATCAA546270.20244039492831353No Hit
GGGGCGCTTGAGAGAACCATGTCGAAGGAACTGTAGGCACCATCAATAGA544290.20170663326840532No Hit
CATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCA522730.19371678408273807No Hit
AGCGCGCGACCTATACCCGGCCGTCGGGGCCTGTAGGCACCATCAATAGA503290.18651257869454832No Hit
TCTGATGATTCATGATAACTCGACGGATCGCACTGTAGGCACCATCAATA493280.1828030058583457No Hit
ATTGGATGGATGCCCGGGCATTGAGACTGTAGGCACCATCAATAGATCGG454590.16846500655438162No Hit
AAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGTAGGCACCATCAA427750.15851845960895913No Hit
TTGGTTGATGCCATGCTTCCAAGAAAAGCTCCTGTAGGCACCATCAATAG412420.1528373655451243No Hit
TAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAA410850.15225554443095468No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCCTGTAGGCACCATCAATAGATCGG408870.15152178277104644No Hit
TCGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGCTGTAGGCACCATCAAT402590.14919449831436787No Hit
AAGTTCCCTGGAAAGGGGCGCCCTGTAGGCACCATCAATAGATCGGAAGA398960.14784926860453615No Hit
AAAGCGATCTGCCGAAGCTGTGGGCTGTAGGCACCATCAATAGATCGGAA393800.14593704124841173No Hit
CGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCCTGTAGGCACCATCAATA390700.14478822248794934No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATC380170.14088594456934658No Hit
AAAAATGCCCTGCTTCCAGGAAAAGCCTCTGTAGGCACCATCAATAGATC364110.13493432221675772No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGCTGTAGGCACCATCAATA361960.13413756081837253No Hit
AAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAGATCGGAA360950.13376326825447996No Hit
ACGAGCGCAACCCTTATCCTTTGTTGCCAGCTGTAGGCACCATCAATAGA356840.13224015693012503No Hit
TCCTGCGGAAAAATAGCTCGACGCCAGGCTGTAGGCACCATCAATAGATC347570.12880481824964565No Hit
TGAAGTGATAAATGCCCTGCTTCCAGGAAAAGCTGTAGGCACCATCAATA345590.12807105658973744No Hit
AGGGCGGGACACGTGGTATCCTGTCTGACTGTAGGCACCATCAATAGATC345110.12789317497521424No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC333920.12374630983664206Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGATC325320.12055926424310133No Hit
GTAGGGCTACACACGTGCTACAATGGCGCACTGTAGGCACCATCAATAGA316130.11715357249837581No Hit
CAAGCGGTGGAGCATGTGGTTTAATTCGCTGTAGGCACCATCAATAGATC299710.11106853893489456No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACCTTGCTGTAGGCACCATCAATAG292890.10854113766187735No Hit
AAAAAACGCGGCGTGTTCGAATTCTGTAGGCACCATCAATAGATCGGAAG285040.10563203209102913No Hit
TAGAAATCACATTGCGTTAGCATCTGTAGGCACCATCAATAGATCGGAAG284530.10544303287559824No Hit
TAGGTGCGTGCCGCACTCACGAGGGACTGCCACTGTAGGCACCATCAATA278190.10309351321710425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)