FastQCFastQC Report
Sat 2 Sept 2023
SRR12343757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27846655
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51-75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCC6672022.3959861606358106RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGC5091471.828395546969645No Hit
CGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTC4309291.5475072320176337RNA PCR Primer, Index 1 (100% over 26bp)
AACGTCCGTCGTGAAGAGGGAAACTGGAATTCTCGGGTGCCAAGGAACTC4094391.4703345877628748RNA PCR Primer, Index 1 (100% over 26bp)
CGCCCCTCCCACGGCGCGACCGCTCTTGGAATTCTCGGGTGCCAAGGAAC3009601.0807761291257423RNA PCR Primer, Index 1 (100% over 24bp)
CTTCTGGCGCCAAGCGTCCGTCCCTGGAATTCTCGGGTGCCAAGGAACTC2439550.8760657249497291RNA PCR Primer, Index 1 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT2089300.7502876018681598No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGG1502080.5394112865620664No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGG1405000.5045489305627552No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC1390510.4993454330511151No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCA1369790.49190468298616113No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGTGGAATTCTCGGGTGC1359920.4883602716376527No Hit
AACCTCGGGCCGATCGCACGCCCCTGGAATTCTCGGGTGCCAAGGAACTC1327200.476610206863266RNA PCR Primer, Index 1 (100% over 26bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC1183750.42509594060758826No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA1129740.4057004333195495No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG1113350.39981462764558257No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCTGGAATTCTCGGGTGCCAAGGA1110220.39869061472553885RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAA1102010.3957423252451686No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGTGGAATTCTCGGGTG1098480.3944746685014771No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTGGAATTCTCGGGTGCCAAG1027130.368852201458308No Hit
AGTGCGCCCCGGGCGTCGTCGCGCTGGAATTCTCGGGTGCCAAGGAACTC913050.327884982953967RNA PCR Primer, Index 1 (100% over 26bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTTGGAATTCTCGGGTGCCAA907240.3257985564154833No Hit
CGCCCCTCCCACGGCGCGACCGCTCTCTGGAATTCTCGGGTGCCAAGGAA902020.3239240045168801RNA PCR Primer, Index 1 (100% over 23bp)
GTCGCGCCGTCGGGTCCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA878260.3153915613922031RNA PCR Primer, Index 1 (100% over 28bp)
CGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG813250.292045848953851RNA PCR Primer, Index 1 (100% over 29bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTTGGAATTCTCGG808250.290250301158254No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGTGGAATTCTCGGGTGC793970.285122216654029No Hit
AGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCCAAGGAACT788190.2830465634023189RNA PCR Primer, Index 1 (100% over 25bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCTGGAATTCTCGGGTGCCA738700.2652742313214998No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGAATTCTCGGG661750.2376407507472621No Hit
AGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGGTGCCAAGGAACTC646440.2321427833971441RNA PCR Primer, Index 1 (100% over 26bp)
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGT642220.23062734105766025No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC639610.2296900651083586RNA PCR Primer, Index 1 (100% over 26bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA625020.22445065664080657No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGGAATTCTCGGGTGCCAAGG615560.2210534802115371Illumina Small RNA Adapter 2 (100% over 21bp)
AGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCC586610.21065725847503047RNA PCR Primer, Index 1 (100% over 27bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCGGGTGCCAAGGAA574720.20638744581710083RNA PCR Primer, Index 1 (100% over 23bp)
CGGGTCGGGGGTCGGCGCGCGGCGCGGGTGGAATTCTCGGGTGCCAAGGA570830.20499050963212637RNA PCR Primer, Index 1 (100% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTTGGAATTCTCGGGTGCCAAGGAAC568850.20427947270506996RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCA557120.2000671175765994No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCTGGAATTCTCGGGTGCC556300.19977264773812153No Hit
GTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAAC539900.19388325096856338RNA PCR Primer, Index 1 (100% over 24bp)
TGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCAA535670.1923642175334883No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCTGGAATTCTCGGGTGCCAAG527560.18945183900902998No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCTGGAATTCTCGGGTGCCAAGGAA525740.18879825961143268RNA PCR Primer, Index 1 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAA517780.18593974752084227No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA508380.1825641176651199RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG501000.17991388911881875No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG498460.1790017508386555No Hit
CCCGTCCCCGCCCCTTGCCTCTCTGGAATTCTCGGGTGCCAAGGAACTCC492260.1767752715721152RNA PCR Primer, Index 1 (100% over 27bp)
TGGCGCGCGGGCGGGGCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCC482290.1731949492676948RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTGGAATTCTCGGGT474460.17038312141978992No Hit
CGCCCCGGGCGTCGTCGCGCCGTCTGGAATTCTCGGGTGCCAAGGAACTC467170.1677652127338095RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG462180.1659732560338037RNA PCR Primer, Index 1 (100% over 29bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC450220.1616783057067357No Hit
GCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT430370.15454998095821562RNA PCR Primer, Index 1 (100% over 30bp)
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT426780.15326077764097698No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT415470.1491992485273366No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAAG410210.14731033224636855No Hit
AGATAACCTCGGGCCGATCGCACGCCCCTGGAATTCTCGGGTGCCAAGGA403050.14473910780307364RNA PCR Primer, Index 1 (100% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA402450.14452364206760202No Hit
AGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCAAGGAACTCCAG395720.14210683473472846RNA PCR Primer, Index 1 (100% over 29bp)
CGTCGCGCCGTCGGGTCCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCC391810.14070271635857162RNA PCR Primer, Index 1 (100% over 27bp)
CCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAAGGAA384960.13824281587860374RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCTGGAATTCTCGGGTGCCAAGGAACTCC379950.13644367698741555RNA PCR Primer, Index 1 (100% over 27bp)
CCGCGCAGTCCGCCCGGAGGATTCTGGAATTCTCGGGTGCCAAGGAACTC366020.13144128082888232RNA PCR Primer, Index 1 (100% over 26bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG354230.1272073791268646No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGG351700.12629883194229252Illumina Small RNA Adapter 2 (100% over 21bp)
TGGCGCGCGGGCGGGGCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA350310.12579966965511657RNA PCR Primer, Index 1 (100% over 28bp)
CCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCAAGGA348150.12502399300741868RNA PCR Primer, Index 1 (100% over 22bp)
CCCGTCCCCGCCCCTTGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA343130.12322126302063928RNA PCR Primer, Index 1 (100% over 28bp)
CCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACT323620.1162150355222198RNA PCR Primer, Index 1 (100% over 25bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA319980.11490787672702521No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCTGGAATTCTCGGGTGCCAAGGAAC319270.11465290894005042RNA PCR Primer, Index 1 (100% over 24bp)
CTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCCAAGGAACTCC307720.11050519353222137RNA PCR Primer, Index 1 (100% over 27bp)
GCCCCTCCCACGGCGCGACCGCTCTTGGAATTCTCGGGTGCCAAGGAACT306230.10997012028913346RNA PCR Primer, Index 1 (100% over 25bp)
CGCCCCTCCCACGGCGCGACCGCTCTGGAATTCTCGGGTGCCAAGGAACT302500.10863064163361813RNA PCR Primer, Index 1 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCG300820.10802733757429753No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT289060.10380420915905339No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAG286100.10274124486405999No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGG283880.1019440216428149No Hit
CGGGGGTCGGCGCGCGGCGCGGGTGGAATTCTCGGGTGCCAAGGAACTCC282700.10152027236305403RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)