FastQCFastQC Report
Sat 2 Sept 2023
SRR12343758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12343758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27680325
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51-75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGC9163173.3103549181593785No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT4443541.6053062960785323No Hit
CTTCTGGCGCCAAGCGTCCGTCCCTGGAATTCTCGGGTGCCAAGGAACTC3474451.2552056379395835RNA PCR Primer, Index 1 (100% over 26bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGG3031161.0950593968820814No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGG2550040.9212464087759085No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC2520690.910643209572142No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCA2155140.7785818988758261No Hit
AACCTCGGGCCGATCGCACGCCCCTGGAATTCTCGGGTGCCAAGGAACTC2128700.7690299879065726RNA PCR Primer, Index 1 (100% over 26bp)
ATGCGTGCATTTATCAGATCAAAACCAACCTGGAATTCTCGGGTGCCAAG1838600.664226305146345No Hit
GTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTCC1795090.6485075590694835RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAA1416840.5118581519545019No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1288120.4653558077804361No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA1207250.43614011034913786No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATTGGAATTCTCGGGTGCC1194850.43166039416083446No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGTGGAATTCTCGGGTG1148890.41505654286934857No Hit
ATGCGTGCATTTATCAGATCAAAACCAATGGAATTCTCGGGTGCCAAGGA1142400.41271191721918RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG1124350.40619104002572226No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC1030510.3722897039684325No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGTGGAATTCTCGGGTGCCAAGGAA946260.341852922608387RNA PCR Primer, Index 1 (100% over 23bp)
CGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGTGCCAAGGAACTC925900.33449751764114044RNA PCR Primer, Index 1 (100% over 26bp)
AGATAACCTCGGGCCGATCGCACGCCCCTGGAATTCTCGGGTGCCAAGGA919260.3320987018757908RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGG889930.3215027280207151No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGTGGAATTCTCGGGTGC853510.30834536805474644No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTGGAATTCTCGGGTGCCAAG828000.2991294357996158No Hit
AGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGGTGCCAAGGAACTC822940.29730142258084036RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAA821770.2968787396824279No Hit
CTTCTGGCGCCAAGCGTCCGTCCTGGAATTCTCGGGTGCCAAGGAACTCC821240.29668726794212136RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA820690.296488570853124RNA PCR Primer, Index 1 (100% over 22bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTTGGAATTCTCGGGTGCCAA762660.27552422162673307No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCA744880.2691008866406012No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA732860.2647584520774232No Hit
AGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGGTGCCAAGGAACT708210.25585320981599746RNA PCR Primer, Index 1 (100% over 25bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGTGGAATTCTCGGGTGC705980.2550475834369719No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCTGGAATTCTCGGGTGCCAAGG698030.25217550733237415Illumina Small RNA Adapter 2 (100% over 21bp)
ATGCGTGCATTTATCAGATCAAAACTGGAATTCTCGGGTGCCAAGGAACT692800.25028607864972685RNA PCR Primer, Index 1 (100% over 25bp)
CGCCCCTCCCACGGCGCGACCGCTCTTGGAATTCTCGGGTGCCAAGGAAC688300.24866037519429415RNA PCR Primer, Index 1 (100% over 24bp)
ATGCGTGCATTTATCAGATCAAAACCAACTGGAATTCTCGGGTGCCAAGG664150.23993576665013866Illumina Small RNA Adapter 2 (100% over 21bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCTGGAATTCTCGGGTGCCA654570.23647482462723973No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTTGGAATTCTCGG621070.2243723655701297No Hit
AGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCC617950.22324521117436302RNA PCR Primer, Index 1 (100% over 27bp)
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGG616710.22279723955553268No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCTGGAATTCTCGGG593450.21439415902811837No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGT591030.21351989183653008No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCCAA585400.21148595618006652No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAAG565600.20433286097616266No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCG561190.20273967158983863No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCGGGT560660.20254819984953212No Hit
AACGTCCGTCGTGAAGAGGGAAACTGGAATTCTCGGGTGCCAAGGAACTC553370.19991456025173115RNA PCR Primer, Index 1 (100% over 26bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC538190.19443052059540486No Hit
CTTTGGTGACTCTAGATAACCTCTGGAATTCTCGGGTGCCAAGGAACTCC510560.18444870137904812RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA452680.16353854226783826No Hit
ATGCGTGCATTTATCAGATCAAAACCTGGAATTCTCGGGTGCCAAGGAAC445360.16089406464700107RNA PCR Primer, Index 1 (100% over 24bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAA443670.16028352268262747No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT433790.15671420042936635No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTTGGAATTCTCGGGTGCCA425660.15377709618655128No Hit
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCTGGAATTCTCGGGT419240.15145775925680063No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT406320.1467901840025361No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC397520.14361103057857882RNA PCR Primer, Index 1 (100% over 26bp)
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCTGGAATTCTCGGGTGCC388750.14044271517765777No Hit
GGGGATGCGTGCATTTATCAGATCTGGAATTCTCGGGTGCCAAGGAACTC380440.13744058279662538RNA PCR Primer, Index 1 (100% over 26bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA373630.13498035156740393No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTTGGAATTCTCGGG373250.1348430699422785No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC366080.1322527824366224No Hit
CGGGTCGGGGGTCGGCGCGCGGCGCGGGTGGAATTCTCGGGTGCCAAGGA356290.12871597425246994RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGGAATTCTCGGGTG345260.12473119444948713No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG341670.12343424435948638No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGGAATTCTCGGGTGCCAAGGA336620.12160984381505636RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA336450.12154842835118446No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGG330000.11921825339839759Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATTGGAATTCTCGGGTGC328360.11862577480575101No Hit
GGGGATGCGTGCATTTATCAGATCAAAATGGAATTCTCGGGTGCCAAGGA327610.11835482422984556RNA PCR Primer, Index 1 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC327020.11814167644346663No Hit
CCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAAGGAA323670.11693143053775559RNA PCR Primer, Index 1 (100% over 23bp)
AGATAACCTCGGGCCGATCGCACGCCTGGAATTCTCGGGTGCCAAGGAAC317670.11476382593051201RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG314480.1136113828143275No Hit
CGTGCATTTATCAGATCAAAACCAACCTGGAATTCTCGGGTGCCAAGGAA314210.11351384060700155RNA PCR Primer, Index 1 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA308930.11160634855262717No Hit
GGGATGCGTGCATTTATCAGATCAAAACCAATGGAATTCTCGGGTGCCAA302630.1093303637150214No Hit
GGGATGCGTGCATTTATCAGATCAAAATGGAATTCTCGGGTGCCAAGGAA301480.10891490616529971RNA PCR Primer, Index 1 (100% over 23bp)
TGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG298780.1079394840920401RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA293740.1061186962219555No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA287250.103774070571787No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCC282100.10191354328390291No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCTGGAATTCTCGGGTGCCAAGGA281050.10153421247763529RNA PCR Primer, Index 1 (100% over 22bp)
TGGCGCGCGGGCGGGGCCGGGGTGGAATTCTCGGGTGCCAAGGAACTCCA277590.10028422715412481RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)