FastQCFastQC Report
Sat 2 Sept 2023
SRR12354645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12354645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52634336
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCT28309745.378568849049412Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC14001392.66012475202499Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10612682.0163035779533725Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCA8680871.6492789041738838Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCC4474260.8500648702018393Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG3994740.7589608425952215TruSeq Adapter, Index 2 (100% over 49bp)
GTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA3543300.6731917355241263Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3168240.6019340682857669No Hit
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTC2992030.5684559220049816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCT2545870.4836899623850104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACT2386570.45342454780848757Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAAC1936430.36790242779922216Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGCAGTGGCTGGTTGAGATTTAAGATCGGAAGAGCACACGTCTGAACT1715140.32585953017437136Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTC1673190.31788944767917277Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA1333420.25333652921925337Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT1270890.24145645154524226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC1249190.237333667513161No Hit
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG1192070.22648143599645676Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGGCTGGTTGAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1166560.22163478988316676Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACTCCAG1122550.21327332788999182Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1086120.20635199045733188Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCAGTGGCTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG987250.18756767445494135TruSeq Adapter, Index 2 (100% over 35bp)
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG821240.15602742665928188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC794470.15094139308606458No Hit
AGCAGTGGCTGGTTGAGATTCAGATCGGAAGAGCACACGTCTGAACTCCA786320.1493929741984396Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGAGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACT780890.14836132824018147Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG747050.14193206503070543No Hit
GCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG721100.13700182329648844Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA714950.13583338450398613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTG696900.1324040641455038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC689500.13099813779354982No Hit
AGAGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCC653420.12414329687753638Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGCAGTGGCTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC619660.11772923287186525Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGAGCAGTGGCTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC609990.115892029111947Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT569190.10814043517144396Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC556470.10572376176646361Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG537560.10213104996707853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)