FastQCFastQC Report
Sat 2 Sept 2023
SRR12354647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12354647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41084822
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCT19309894.700005758817697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC2854320.6947383147966419Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2561530.6234735542970102No Hit
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCA2060110.501428483735429Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1993060.4851085882762252Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1855470.45161933523771874TruSeq Adapter, Index 7 (100% over 49bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCT1736070.4225575079770335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAAC1676240.4079949524912144Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC1264110.3076829686641943No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC1259280.30650735203379975No Hit
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTC1083140.263635071852082Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC944010.22977098452562358No Hit
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCC938650.2284663664844404Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC714000.17378680623223827No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC545700.13282277333463924No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG541080.13169827047078358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTG501050.12195501297291733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT481780.11726471639575316No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG469200.11420275838118514No Hit
TGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGAGATCGGAAGAGCAC465990.11342144794980492No Hit
AGAGAGCAGTGGCTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC428880.10438891520571758Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA427170.10397270310675802Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG419190.10203037997828004No Hit
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA415870.10122229566918897Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)