FastQCFastQC Report
Sat 2 Sept 2023
SRR12354648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12354648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53748519
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCT32610516.067238801500745Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC9543241.775535433822837Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCA9129161.6984951715599828Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA6124511.1394751174446314No Hit
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4944700.919969534416381Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCT2881400.5360891897319069Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1760570.32755693231286986TruSeq Adapter, Index 10 (100% over 49bp)
GCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACT1653050.30755266019515815Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA1649730.3069349687569996Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA1505230.28005050706606444Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC1487230.27670157758207253No Hit
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCC1426610.2654231272865397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGCAGTGGCTGGTTGAGATTTAAGATCGGAAGAGCACACGTCTGAACT1405970.261583021478229Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1399630.26040345409331184No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG1170290.21773437143449478Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA1081610.20123531217669458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTC1075600.2001171418323173Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC930330.17308942038012248No Hit
CAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTC914300.17010701262298966Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAAC828430.1541307584679682Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTG806490.15004878553025805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGTGGCTGGTTGAGATTCAGATCGGAAGAGCACACGTCTGAACTCCA736810.13708470739444933Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC592330.11020396673627417No Hit
GCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG562010.10456288107212777Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGGCTGGTTGAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA541770.10079719591901685Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)