FastQCFastQC Report
Sat 2 Sept 2023
SRR12354649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12354649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51448991
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCA9753621.8957845062500835Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9155121.77945569428174Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCT7703811.4973685295402586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC4910370.9544152187552134Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTC4169070.8103307604224931Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAAC2236360.4346751912005427Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1396240.2713833591022222TruSeq Adapter, Index 4 (100% over 49bp)
CAGTGGCTGGTTGAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1236430.2403215254503242Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCAGTGGCTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1140490.22167392942652656TruSeq Adapter, Index 4 (100% over 35bp)
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCC990230.19246830321706407Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCAGTGGCTGGTTGAGATTCAGATCGGAAGAGCACACGTCTGAACTCCA943030.18329416800418885Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGCAGTGGCTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC866430.16840563501041256Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA848060.16483510823370665No Hit
GTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA826660.16067564862447933Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC822640.15989429219321327Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC760540.14782408463559568No Hit
AGAGCAGTGGCTGGTTGAGATTTAAGATCGGAAGAGCACACGTCTGAACT758900.14750532231040256Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCT751610.14608838490146484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG729170.14172678332991992Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG710080.13801631211776338Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA662120.12869445777857919Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACT659790.1282415820360792Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGCAGTGGCTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC596100.11586233051684143Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC518080.10069779599759304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)