FastQCFastQC Report
Sat 2 Sept 2023
SRR12354650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12354650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54183253
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCT27540765.082891571681752Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCA8580291.5835686351279057Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC6934961.2799083879293847Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5529801.0205736447754439Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG3191460.5890122543952834TruSeq Adapter, Index 6 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2454720.45304035178544927No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCT2281960.42115596123399973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTC1784080.3292677905477547Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAAC1712570.31606998568358385Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCC1323110.24419168778958325Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA1257630.23210677291745477Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACT1239680.22879394118326563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA1137310.2099006495604832Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1029150.18993876207469493No Hit
AGAGCAGTGGCTGGTTGAGATTTAAGATCGGAAGAGCACACGTCTGAACT933790.17233922813752064Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG890560.16436074814481885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC785520.14497468433650523No Hit
AGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA778690.14371414724767448Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAGTGGCTGGTTGAGATTCAGATCGGAAGAGCACACGTCTGAACTCCA699950.1291819817462787Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTC680660.125621841124969Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTG655860.12104478112452938Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG653070.12052986187447992No Hit
ACGAGCTGTGGGGATCTGGCACTGTGGTTCCTGCAGATCGGAAGAGCACA648830.11974733226150153No Hit
GCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG648120.11961629546310185Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAG597000.11018164597832471Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCAGATCGGAAGAGCA592060.1092699251556565No Hit
CAGTGGCTGGTTGAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA583340.1076605718006632Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCAGATCGGAAGAGCACA558820.10313518828409952No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)