FastQCFastQC Report
Sat 2 Sept 2023
SRR12354651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12354651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44183331
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCT20736364.693254114317456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCA6208911.405260730568277Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC5215231.180361435401962Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2558990.5791754360937613Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2489710.5634953145565236No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCT1566950.3546473216335817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAAC1305700.29551868780558893Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTC1286110.29108488900486024Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG975780.22084799355666507TruSeq Adapter, Index 9 (100% over 49bp)
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCC836750.18938137552372408Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA786520.17801283474982907Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA777020.17586270261062933Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGCAGTGGCTGGTTGAGATTTAAGATCGGAAGAGCACACGTCTGAACT769980.1742693415306329Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACT744220.16843908848791866Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG701830.15884497255311059Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC636910.14415164850291617No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC600530.13591777405827551No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC582220.1317736772720916No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTG551180.12474840341938004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA493290.11164617715219345Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC479080.10843003213134834No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA469120.10617578833067157No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC467610.10583403048538827No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC466060.1054832194521504No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT465630.10538589768163925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGTGGCTGGTTGAGATTCAGATCGGAAGAGCACACGTCTGAACTCCA444070.10050622937415017Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)