FastQCFastQC Report
Sat 2 Sept 2023
SRR12354652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12354652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71115943
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCT51011497.17300338687768Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCA9610261.3513509903116943Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAAC8894431.2506942360308715Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA7932041.115367337532176No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCT4533710.6375096509653257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2326120.3270883998543055Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2205630.3101456448380358TruSeq Adapter, Index 12 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1749070.24594625708612203No Hit
GCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACT1632270.22952237306337903Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA1589400.2234941889190726Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC1583800.2227067424248315No Hit
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA1574130.22134699106781164Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1481750.20835693622174145No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG1449020.203754592693793Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1402050.19714988522334578No Hit
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCC1343500.18891685089516425Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGCAGTGGCTGGTTGAGATTTAAGATCGGAAGAGCACACGTCTGAACT1341830.1886820231013459Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTG1224260.17214986518564482Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA1176110.16537923148962533Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1059470.1489778459381464No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1006000.14145913807259788No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG927870.13047285332348052No Hit
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTC926500.13028021016328223Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAAC913230.12841424320282163Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG892870.12555131273447362No Hit
CAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTC856370.12041884897736645Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGAGCTGTGGGGATCTGGCACTGTGGTTCCTGCAGATCGGAAGAGCACA775060.10898540711187645No Hit
AGCAGTGGCTGGTTGAGATTCAGATCGGAAGAGCACACGTCTGAACTCCA761950.10714193862267987Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA725660.10203900410910673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)