FastQCFastQC Report
Tue 18 Jul 2023
SRR12381559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12381559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29945301
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC2233478474.58527132520724RNA PCR Primer, Index 8 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCTTGAATCTCGTATGC3187051.0642905209067692RNA PCR Primer, Index 8 (98% over 50bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC2644280.883036707495443RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGTATCTCGTATGC2441930.8154635012685296RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTTAATCTCGTATGC2240360.7481507699655449RNA PCR Primer, Index 8 (98% over 50bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC2016110.673264229336015RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGGAATCTCGTATGC1892170.6318754318081491RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTTGAATCTCGTATGC1330180.4442032491174492RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACGTGAATCTCGTATGC1074330.3587641346467013RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGCATCTCGTATGC1006510.3361161739533024RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAAACTTGAATCTCGTATGC841370.28096895736663324RNA PCR Primer, Index 8 (98% over 50bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC787330.26292272032930974RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGTTGAATCTCGTATGC749710.25035981438289767RNA PCR Primer, Index 14 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGGATCTCGTATGC725740.24235521960523954RNA PCR Primer, Index 8 (98% over 50bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATG685070.22877378991782382RNA PCR Primer, Index 8 (100% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTTTGAATCTCGTATGC649270.21681865879391227RNA PCR Primer, Index 21 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTTGTATCTCGTATGC636700.21262100521213662RNA PCR Primer, Index 8 (97% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCTTGAATCTCGTATGC616440.20585533603419115RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTGAATCTCGTATGC612240.2044527787514976RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTTGATCTCGTATGC529620.17686247334765479RNA PCR Primer, Index 8 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGGGATCTCGTATGC502940.16795289518044917RNA PCR Primer, Index 8 (97% over 38bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC502120.16767906256811377RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAATTGAATCTCGTATGC460910.15391730408720888RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTTGAATCTCGTATGC456160.1523310785889245RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATTTCGTATGC440760.14718836855238157RNA PCR Primer, Index 8 (98% over 50bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC437000.14593274584216068RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTCAATCTCGTATGC421950.1409069155791755RNA PCR Primer, Index 8 (98% over 50bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCC383410.1280367827994115RNA PCR Primer, Index 8 (100% over 50bp)
TCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTAT366550.12240651713602746RNA PCR Primer, Index 8 (100% over 48bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGAATGC358470.11970826407789323RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGTGATCTCGTATGC337610.11274222957384868RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGCATGC331020.11054155040886049RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGTCAAGGAACTCCAGTCACACTTGAATCTCGTATGC320480.10702179951372004RNA PCR Primer, Index 8 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGGATGC317300.10595986328539493RNA PCR Primer, Index 8 (98% over 50bp)
TGGANTTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGC311230.10393283406969261RNA PCR Primer, Index 8 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)