FastQCFastQC Report
Tue 18 Jul 2023
SRR12381560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12381560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25747510
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC1842987071.57923232188278RNA PCR Primer, Index 7 (100% over 50bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC2088400.8111075595271154RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATTATCTCGTATGC2038760.7918280253119623RNA PCR Primer, Index 7 (98% over 50bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC1526250.5927757674431431RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGTTCATCTCGTATGC1490260.5787977167500857RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGATCATCTCGTATGC1404410.5454546866862077RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTGATCATCTCGTATGC1109190.4307950555218738RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAGCATCTCGTATGC1042310.40481972819895984RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGATCATCTCGTATGC887260.3446003128069472RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATAATCTCGTATGC795970.3091444570756551RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGTCATCTCGTATGC759260.2948867676913224RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGCTCATCTCGTATGC686350.26656946632897704RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAACAGATCATCTCGTATGC669510.2600290280497026RNA PCR Primer, Index 7 (98% over 50bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC650600.25268462853301155RNA PCR Primer, Index 7 (98% over 50bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATG634780.2465403450663773RNA PCR Primer, Index 7 (100% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGATCATCTCGTATGC545600.2119039860553506RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGTGCATCTCGTATGC508840.1976268773174571RNA PCR Primer, Index 23 (97% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATGATCTCGTATGC493100.19151366481652013RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATATCATCTCGTATGC490410.19046890359494958RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTGATCATCTCGTATGC466200.1810660526008146RNA PCR Primer, Index 19 (97% over 37bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC412290.1601281055915698RNA PCR Primer, Index 7 (98% over 50bp)
TCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTAT359170.13949698436858554RNA PCR Primer, Index 7 (100% over 48bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC345800.13430424922643006RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGATTATCTCGTATGC344000.1336051524982416RNA PCR Primer, Index 7 (97% over 38bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC328370.12753466257513835RNA PCR Primer, Index 7 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGATTATCTCGTATGC312630.12142145007420135RNA PCR Primer, Index 7 (97% over 38bp)
GATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGT309990.1203961082061916RNA PCR Primer, Index 7 (100% over 46bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGAATGC301670.11716472777367598RNA PCR Primer, Index 7 (98% over 50bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC297480.11553738594528169RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATTTCGTATGC293580.11402267636754002RNA PCR Primer, Index 7 (98% over 50bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA281600.1093697992543745No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGCATGC272860.10597529625194825RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGTCAAGGAACTCCAGTCACCAGATCATCTCGTATGC272730.1059248059326902RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGGATGC265420.10308569644210255RNA PCR Primer, Index 7 (98% over 50bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC262820.10207589005694143RNA PCR Primer, Index 7 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGATCATCTCGTATGC257880.10015725792513529RNA PCR Primer, Index 7 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)