SRR12381561.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR12381561.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19958828
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	65
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC	11158116	55.9056674069239	RNA PCR Primer, Index 6 (100% over 50bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA	253626	1.270745957628374	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCATTATCTCGTATGC	180119	0.9024527893120778	RNA PCR Primer, Index 6 (98% over 50bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC	137825	0.6905465591466593	RNA PCR Primer, Index 6 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCAATATCTCGTATGC	137050	0.6866635656161775	RNA PCR Primer, Index 6 (98% over 50bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC	114621	0.5742872276869162	RNA PCR Primer, Index 1 (100% over 24bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC	110901	0.5556488587406034	RNA PCR Primer, Index 6 (98% over 50bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA	78901	0.3953188032884496	No Hit
GTGGTGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA	69686	0.34914875763246217	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCAATATCTCGTATGC	60017	0.3007040293147473	RNA PCR Primer, Index 6 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTAATATCTCGTATGC	58105	0.29112430850148113	RNA PCR Primer, Index 6 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCTATATCTCGTATGC	53098	0.266037665137452	RNA PCR Primer, Index 6 (98% over 50bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG	52541	0.2632469201097379	Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAGATCTCGTATGC	46434	0.23264893108954093	RNA PCR Primer, Index 6 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACAATATCTCGTATGC	43183	0.2163603995184487	RNA PCR Primer, Index 6 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCACTATCTCGTATGC	41654	0.20869962905637549	RNA PCR Primer, Index 6 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAAGCCAATATCTCGTATGC	40835	0.20459618169964688	RNA PCR Primer, Index 6 (98% over 50bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC	39563	0.19822306199542378	RNA PCR Primer, Index 6 (98% over 50bp)
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA	38731	0.19405448055366778	RNA PCR Primer, Index 6 (100% over 38bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATG	38123	0.19100820950007685	RNA PCR Primer, Index 6 (100% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCAAATATCTCGTATGC	36710	0.1839286354890177	RNA PCR Primer, Index 6 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCCATATCTCGTATGC	36228	0.18151366402876962	RNA PCR Primer, Index 6 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCAATATCTCGTATGC	34448	0.17259530469424358	RNA PCR Primer, Index 6 (98% over 50bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC	32053	0.16059560210649643	RNA PCR Primer, Index 6 (98% over 50bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG	30114	0.15088060280894247	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTTATATCTCGTATGC	27429	0.1374279090936602	RNA PCR Primer, Index 8 (97% over 37bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA	26207	0.13130530510108107	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAGTATCTCGTATGC	25807	0.12930117940792915	RNA PCR Primer, Index 6 (98% over 50bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG	25146	0.1259893616999956	No Hit
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC	25136	0.1259392585576668	RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACATTATCTCGTATGC	24676	0.1236345140105421	RNA PCR Primer, Index 6 (97% over 37bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC	24588	0.12319360635804869	RNA PCR Primer, Index 6 (98% over 50bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC	23883	0.11966133482386843	RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATTTCGTATGC	23758	0.11903504554475844	RNA PCR Primer, Index 6 (98% over 50bp)
TGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCCA	23021	0.11534244395512604	No Hit
TTGAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC	22746	0.11396460754108408	RNA PCR Primer, Index 1 (100% over 24bp)
TCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTAT	21691	0.10867872602539588	RNA PCR Primer, Index 6 (100% over 48bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCGATATCTCGTATGC	20797	0.10419950510120134	RNA PCR Primer, Index 6 (98% over 50bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA	20614	0.10328261759658434	No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG	19966	0.1000359339736782	No Hit

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content