FastQCFastQC Report
Tue 18 Jul 2023
SRR12381562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12381562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24901892
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1525513861.26095960901284RNA PCR Primer, Index 5 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGTGATCTCGTATGC2152130.8642435683200297RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCAGTGATCTCGTATGC1981020.7955299139519197RNA PCR Primer, Index 5 (98% over 50bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1703010.6838877945499081RNA PCR Primer, Index 5 (98% over 50bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1260990.5063832097577164RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATAGTGATCTCGTATGC1131070.4542104672207236RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGGGATCTCGTATGC1126220.4522628240456589RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACGGTGATCTCGTATGC919430.36922094112367043RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACCGTGATCTCGTATGC725640.29139954506268034RNA PCR Primer, Index 5 (98% over 50bp)
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGT714040.2867412644790203RNA PCR Primer, Index 5 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGGGATCTCGTATGC665810.2673732582247164RNA PCR Primer, Index 8 (97% over 38bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA638290.2563218891158953No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGTGATCTCGTATGC621660.24964368169294124RNA PCR Primer, Index 3 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAAACAGTGATCTCGTATGC578920.2324803271976282RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACATTGATCTCGTATGC542380.21780674335909897RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCAGTGATCTCGTATGC507690.20387607495848106RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTTGATCTCGTATGC503080.20202481000238856RNA PCR Primer, Index 8 (97% over 37bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC497880.19993661525798923RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAAGTGATCTCGTATGC497590.19982015824339772RNA PCR Primer, Index 5 (98% over 50bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC480040.19277250098104995RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAGTGATCTCGTATGC466230.18722673763102016RNA PCR Primer, Index 5 (98% over 50bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATG434660.17454898607704186RNA PCR Primer, Index 5 (100% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTAATCTCGTATGC401300.16115241363989533RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTTATCTCGTATGC382110.153446171881237RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAGTGATCTCGTATGC357210.1434469316628632RNA PCR Primer, Index 3 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGAGTGATCTCGTATGC337800.13565234320348027RNA PCR Primer, Index 5 (98% over 50bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC321860.12925122316007154RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAATGATCTCGTATGC321500.12910665583161313RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTCATCTCGTATGC315740.12679357857627846RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATTTCGTATGC274640.11028880857727598RNA PCR Primer, Index 5 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGACTCGTATGCC265120.10646580589137565RNA PCR Primer, Index 5 (97% over 40bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC252420.10136579180409264RNA PCR Primer, Index 5 (98% over 50bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA252320.10132563421285418No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)