FastQCFastQC Report
Tue 18 Jul 2023
SRR12381563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12381563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42612026
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC1790171842.010952494959994RNA PCR Primer, Index 3 (100% over 50bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC15499643.637386309676991RNA PCR Primer, Index 3 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGTCATCTCGTATGC4543971.0663585908823017RNA PCR Primer, Index 3 (98% over 50bp)
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG4386291.0293549525197419RNA PCR Primer, Index 3 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTTGGCATCTCGTATGC2221390.5213058867466194RNA PCR Primer, Index 3 (98% over 50bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC2062060.48391503375127015RNA PCR Primer, Index 3 (98% over 50bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC1559470.3659694566036358RNA PCR Primer, Index 3 (98% over 50bp)
TGGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC1289900.30270797262725785RNA PCR Primer, Index 3 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGAATCTCGTATGC1289720.30266573103095357RNA PCR Primer, Index 3 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTATGCATCTCGTATGC1169180.27437794203917926RNA PCR Primer, Index 3 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTGGGCATCTCGTATGC1032520.24230718342282057RNA PCR Primer, Index 3 (98% over 50bp)
CCCGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC1007750.23649427042027996RNA PCR Primer, Index 3 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAGGCATCTCGTATGC995400.23359602756273545RNA PCR Primer, Index 3 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGGATCTCGTATGC972000.2281046200431775RNA PCR Primer, Index 3 (98% over 50bp)
ACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCG963060.2260066207600643RNA PCR Primer, Index 3 (100% over 45bp)
CCCCGCCGGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG874690.20526834373000713RNA PCR Primer, Index 3 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGTGATCTCGTATGC868970.20392599966967073RNA PCR Primer, Index 3 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGTATCTCGTATGC818390.19205611110816465RNA PCR Primer, Index 3 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTTTGCATCTCGTATGC718900.16870824212864227RNA PCR Primer, Index 21 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGCCATCTCGTATGC704620.16535707548850176RNA PCR Primer, Index 3 (98% over 50bp)
TGGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC702060.16475630611884073RNA PCR Primer, Index 3 (100% over 44bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC677010.1588776839664934RNA PCR Primer, Index 3 (98% over 50bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT640980.15042232443958425No Hit
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG601600.14118080187034526RNA PCR Primer, Index 3 (100% over 49bp)
CCCCCGCCGGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA596780.14004966579153033RNA PCR Primer, Index 3 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAATTAGGCATCTCGTATGC592780.1391109636514349RNA PCR Primer, Index 3 (98% over 50bp)
GTCCGGTGCGGAGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC565530.13271605532203515RNA PCR Primer, Index 1 (100% over 31bp)
GAGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG508460.11932312253822429RNA PCR Primer, Index 3 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAAGCATCTCGTATGC503250.11810046300075007RNA PCR Primer, Index 3 (98% over 50bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA485940.11403822948948732No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTGGTCATCTCGTATGC484940.11380355395446345RNA PCR Primer, Index 3 (97% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTACGCATCTCGTATGC484180.11362520054784533RNA PCR Primer, Index 3 (98% over 50bp)
CAAGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC474000.11123620360130261RNA PCR Primer, Index 3 (100% over 44bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTTGGAATCTCGTATGC460390.10804226956962806RNA PCR Primer, Index 3 (97% over 38bp)
TACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC441720.10366087733073288RNA PCR Primer, Index 3 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)