FastQCFastQC Report
Tue 18 Jul 2023
SRR12381564.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12381564.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33767697
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1859052455.05416611621455RNA PCR Primer, Index 4 (100% over 50bp)
GCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC3146660.9318550803153678RNA PCR Primer, Index 4 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACTAATCTCGTATGC3107680.920311503624307RNA PCR Primer, Index 4 (98% over 50bp)
CACGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC3032110.8979321272635205RNA PCR Primer, Index 4 (100% over 44bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC2200140.6515516885856918RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGTCCAATCTCGTATGC1871170.5541301795026176RNA PCR Primer, Index 4 (98% over 50bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1746980.5173524270843819RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTACCAATCTCGTATGC1663520.49263649813015087RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCCATCTCGTATGC1268410.3756282224399254RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGCCAATCTCGTATGC775040.22952113080142836RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCTATCTCGTATGC770900.22829510700714947RNA PCR Primer, Index 4 (98% over 50bp)
CCCCGCCGGGCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC710540.21042003545577895RNA PCR Primer, Index 4 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACGAATCTCGTATGC676170.20024166883515923RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGATCAATCTCGTATGC653160.19342746412347872RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAATGACCAATCTCGTATGC649990.19248869711191732RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAGCAATCTCGTATGC648920.19217182622788875RNA PCR Primer, Index 4 (98% over 50bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC632570.18732992066352644RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGTTCAATCTCGTATGC622690.1844040474539913RNA PCR Primer, Index 14 (97% over 38bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATG589820.17466989235303787RNA PCR Primer, Index 4 (100% over 49bp)
GCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC556630.16484097212788898RNA PCR Primer, Index 4 (100% over 37bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC525760.1556990990531572RNA PCR Primer, Index 4 (100% over 37bp)
CCCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC518970.1536883015741346RNA PCR Primer, Index 4 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCCCAATCTCGTATGC516220.15287391378807977RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGGCTAATCTCGTATGC506750.15006945839392008RNA PCR Primer, Index 10 (97% over 38bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC444150.13153103097318125RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTACCCATCTCGTATGC429020.12705041744481418RNA PCR Primer, Index 4 (97% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC424910.12583327788092863RNA PCR Primer, Index 3 (100% over 50bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC402730.1192648702101301RNA PCR Primer, Index 4 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCACCAATCTCGTATGC399880.11842086832276422RNA PCR Primer, Index 4 (98% over 50bp)
GGAGCGGGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA398930.11813953436030891RNA PCR Primer, Index 4 (100% over 39bp)
CGCTCGGCGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC389380.11531138768510035RNA PCR Primer, Index 4 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)