FastQCFastQC Report
Tue 18 Jul 2023
SRR12381565.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12381565.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633057
Total Bases236.1 Mbp
Sequences flagged as poor quality0
Sequence length65
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1871815.152162490156361RNA PCR Primer, Index 1 (100% over 31bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1845905.080845139506482No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC1114423.0674443037915453No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA785942.16330214472275No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC685551.8869783766123132No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA388571.069540059514618No Hit
ATCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA348300.9586967669375955No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC319030.8781310064774651RNA PCR Primer, Index 7 (100% over 50bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG279960.7705907174041035No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATGGAATTCTCGGGTGCC253570.6979521653527594No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA238080.6553158951263358No Hit
CGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC217130.5976509589582547RNA PCR Primer, Index 1 (100% over 31bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG198710.5469498551770589No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC198240.5456561788047917RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG189970.5228929796587282No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC189620.521929603636827No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC176650.48622964076809144No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA161340.4440888210672169No Hit
GTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA155600.42828945430803866No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA152300.419206194672971No Hit
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT151710.4175822179503377RNA PCR Primer, Index 1 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA145430.40029649961451197No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA139750.3846622830305167No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCC137880.379515102570645No Hit
GCCCCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC112350.309243703030258No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG105480.2903340079717989No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC96010.2642678053220745No Hit
GCCTCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC90030.247807837862164No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC84870.23360492279642184No Hit
CGGGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC82250.2263933651467621RNA PCR Primer, Index 1 (100% over 31bp)
CTAGACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC79860.2198148831686373RNA PCR Primer, Index 1 (100% over 24bp)
GAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAGGAAC78050.2148328528839487RNA PCR Primer, Index 1 (100% over 24bp)
AGGGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC76990.21191519978904816RNA PCR Primer, Index 1 (100% over 31bp)
TCGGTTGGCCCCGGATAGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTC75870.2088323965189646RNA PCR Primer, Index 1 (100% over 26bp)
GCCACCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC69430.1911062777159841No Hit
CGTTTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC65890.18136241738018422RNA PCR Primer, Index 1 (100% over 31bp)
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG64690.17805941387652327No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCA63060.17357283411738378No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA62070.1708478562268635No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATGGAATTCTCGGGTGCCA58110.15994794466478232No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA55980.1540851134457841No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA55900.1538649132122067No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGAA53610.14756168152605367RNA PCR Primer, Index 1 (100% over 23bp)
TGGATAACTGTGGTAATTCTAGAGCTAATACATGTGGAATTCTCGGGTGC53050.14602027989101188No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA52490.1444788782559701No Hit
CGGTTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC51800.14257965124136507RNA PCR Primer, Index 1 (100% over 31bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGATGGAATTCTCGGGTGCCA51720.14235945100778766No Hit
GACGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC49500.13624889452601485No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG48440.13333124143111436Illumina Small RNA Adapter 2 (100% over 21bp)
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA46340.12755098529970765No Hit
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCACGGAACTCCAGTC46330.12752346027051048RNA PCR Primer, Index 1 (96% over 31bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA45960.12650503419021503RNA PCR Primer, Index 1 (100% over 23bp)
CGGCTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC45650.1256517582851026RNA PCR Primer, Index 1 (100% over 31bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATATGGAATTCTCGGGTG44750.12317450565735688No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG40830.11238469421206439No Hit
ATAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC40600.11175161854052935RNA PCR Primer, Index 1 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG39730.10935694100037516Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA39720.10932941597117798RNA PCR Primer, Index 1 (100% over 32bp)
GAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGGAACT39210.10792563948212208RNA PCR Primer, Index 1 (100% over 25bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGCATTCTCGGGTGC39120.1076779142193475No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGGAATTCTCGGGTGCCAAG38100.1048703612412357No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA37700.1037693600733487No Hit
ATTCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA36880.10151230767918037No Hit
AGTTTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC36640.10085170697844818RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)