FastQCFastQC Report
Thu 24 Aug 2023
SRR12381566.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12381566.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62294963
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length65
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC29635824.757338085263812RNA PCR Primer, Index 4 (100% over 50bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA25552794.101903070397522No Hit
AGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT11487681.8440784690730132RNA PCR Primer, Index 1 (100% over 30bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCC10936711.7556331159551373No Hit
AGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC6471141.0387902469739005RNA PCR Primer, Index 1 (100% over 31bp)
GCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCC6231241.000279910271397RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAA6205050.9960757180319699RNA PCR Primer, Index 1 (100% over 23bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAA6057840.9724445939553732No Hit
GGCATTAACGCGAACTCGGCCTACATGGAATTCTCGGGTGCCAAGGAACT5508670.8842881887577332RNA PCR Primer, Index 1 (100% over 25bp)
GGCATTAACGCGAACTCGGCCTACTGGAATTCTCGGGTGCCAAGGAACTC5264450.8450843770466643RNA PCR Primer, Index 1 (100% over 26bp)
GGCATTAACGCGAACTCGGCCTATGGAATTCTCGGGTGCCAAGGAACTCC4560040.7320078189949323RNA PCR Primer, Index 1 (100% over 27bp)
AACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAA4538320.7285211807574233RNA PCR Primer, Index 1 (100% over 23bp)
AACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAAC4306370.6912870307026268RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACAATGGAATTCTCGGGTGCCAAGGAAC4287820.6883092618579773RNA PCR Primer, Index 1 (100% over 24bp)
GGCATTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAG4222520.6778268734183212No Hit
CGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTC4100780.6582843624130574RNA PCR Primer, Index 1 (100% over 26bp)
ACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACT4061170.651925902901652RNA PCR Primer, Index 1 (100% over 25bp)
GGCATTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGA3950010.6340817635608837RNA PCR Primer, Index 1 (100% over 22bp)
AAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTC3862240.6199923419169541RNA PCR Primer, Index 1 (100% over 26bp)
TTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGA3537660.5678886108335918RNA PCR Primer, Index 1 (100% over 22bp)
AGCGTGTACTCCGAAGAGGATCCTGGAATTCTCGGGTGCCAAGGAACTCC3467870.5566854578595705RNA PCR Primer, Index 1 (100% over 27bp)
GGATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAA3241880.5204080464739982No Hit
AAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACT3174330.5095644731340477RNA PCR Primer, Index 1 (100% over 25bp)
AGCGTGTACTCCGAAGAGGATCCAAATGGAATTCTCGGGTGCCAAGGAAC3167320.5084391815113527RNA PCR Primer, Index 1 (100% over 24bp)
GAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG3150000.5056588604121973RNA PCR Primer, Index 1 (100% over 29bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGGAATTCTCGGGTGCCAAGG3079380.4943224703416229Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGTGTACTCCGAAGAGGATCCAAAAGCTGGAATTCTCGGGTGCCAAGG3043510.488564380397818Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAG3009450.4830968436404722RNA PCR Primer, Index 1 (100% over 29bp)
AAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA2974380.47746717499454977RNA PCR Primer, Index 1 (100% over 28bp)
CGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCCA2973080.47725849038549073RNA PCR Primer, Index 1 (100% over 28bp)
ACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAAC2908040.46681783886764644RNA PCR Primer, Index 1 (100% over 24bp)
ATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGG2878770.4621192246313719Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGTGTACTCCGAAGAGGATCCAATGGAATTCTCGGGTGCCAAGGAACT2781960.4465786423213704RNA PCR Primer, Index 1 (100% over 25bp)
AGGATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAA2758840.44286726681256716No Hit
AGCGTGTACTCCGAAGAGGATCCAAAAGCATGGAATTCTCGGGTGCCAAG2700460.43349572259959446No Hit
CGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACT2695080.4326320893713349RNA PCR Primer, Index 1 (100% over 25bp)
AAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCC2661630.4272624738536244RNA PCR Primer, Index 1 (100% over 27bp)
GCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAA2496040.40068087045817813No Hit
AGCGTGTACTCCGAAGAGGATCCAAAATGGAATTCTCGGGTGCCAAGGAA2479420.39801291799467RNA PCR Primer, Index 1 (100% over 23bp)
CAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACT2462910.39536262345962064RNA PCR Primer, Index 1 (100% over 25bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC2390360.3837164170079048RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCA2227960.35764689353776485RNA PCR Primer, Index 1 (100% over 28bp)
GAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCA2224090.3570256555092584RNA PCR Primer, Index 1 (100% over 28bp)
ATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAG2177460.34954029910893436No Hit
AGCGTGTACTCCGAAGAGGATCTGGAATTCTCGGGTGCCAAGGAACTCCA2142990.34400694643642377RNA PCR Primer, Index 1 (100% over 28bp)
AGCGTGTACTCCGAAGAGGATCCAAAAGCAGTTGGAATTCTCGGGTGCCA2135210.3427580493145168No Hit
AAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCC2119670.34026346560314996RNA PCR Primer, Index 1 (100% over 27bp)
GGCATTAACGCGAACTCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2062820.33113752712237743RNA PCR Primer, Index 1 (100% over 29bp)
AAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTC2000830.3211864818027101RNA PCR Primer, Index 1 (100% over 26bp)
AGGATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCA1992550.31985732136962663No Hit
GGATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAG1945680.31233343857993784No Hit
GATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGG1929960.30980996007654743Illumina Small RNA Adapter 2 (100% over 21bp)
TTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGG1927850.30947124890338246Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGTGTACTCCGAAGAGGATCCAAAAGCAGTGGAATTCTCGGGTGCCAA1920380.30827211503440494No Hit
ATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGG1861360.29879783378312624Illumina Small RNA Adapter 2 (100% over 21bp)
TAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAA1850690.2970850147226189RNA PCR Primer, Index 1 (100% over 23bp)
ATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGA1773370.2846730962822789RNA PCR Primer, Index 1 (100% over 22bp)
GATCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAG1755240.28176274861901757No Hit
CAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAAC1719730.27606244825925974RNA PCR Primer, Index 1 (100% over 24bp)
GCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTC1706600.2739547337077638RNA PCR Primer, Index 1 (100% over 26bp)
GCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG1678620.2694631988143247RNA PCR Primer, Index 1 (100% over 29bp)
GGCATTAACGCGAACTCGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA1672420.2684679337557356RNA PCR Primer, Index 1 (100% over 28bp)
AGGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCC1670370.2681388541799118No Hit
AACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACT1669860.2680569856025117RNA PCR Primer, Index 1 (100% over 25bp)
CGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCC1661190.26666521978671054RNA PCR Primer, Index 1 (100% over 27bp)
GCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT1618570.2598235751420223RNA PCR Primer, Index 1 (100% over 30bp)
CCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAAC1613830.2590626789520687RNA PCR Primer, Index 1 (100% over 24bp)
TAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGA1501550.24103874979426507RNA PCR Primer, Index 1 (100% over 22bp)
AACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT1478150.237282426831203RNA PCR Primer, Index 1 (100% over 30bp)
AGCGTGTACTCCGAAGAGGATCCAAAAGTGGAATTCTCGGGTGCCAAGGA1468060.23566271321166046RNA PCR Primer, Index 1 (100% over 22bp)
CATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCAAG1456860.23386481504130602No Hit
ACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACTC1403510.22530071973876925RNA PCR Primer, Index 1 (100% over 26bp)
GAGGATCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCA1402880.22519958796668682No Hit
AACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAG1381630.2217883972416839RNA PCR Primer, Index 1 (100% over 29bp)
AGCGTGTACTCCGAAGAGGATCCATGGAATTCTCGGGTGCCAAGGAACTC1335750.21442343580812465RNA PCR Primer, Index 1 (100% over 26bp)
GGCATTAACGCGAACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1325000.21269777461782904RNA PCR Primer, Index 1 (100% over 30bp)
TCCAAAAGCAGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAA1257040.20178838536271385RNA PCR Primer, Index 1 (100% over 23bp)
TCCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGA1244060.1997047498045709RNA PCR Primer, Index 1 (100% over 22bp)
ACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1243090.19954903898088838RNA PCR Primer, Index 1 (100% over 30bp)
TTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAA1242960.1995281705199825RNA PCR Primer, Index 1 (100% over 23bp)
GCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1184450.19013575784610387RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGAGGTGGGATCCCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1175050.18862680759598494RNA PCR Primer, Index 1 (100% over 27bp)
CGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACTCC1161690.1864821719213478RNA PCR Primer, Index 1 (100% over 27bp)
CATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCAA1146440.18403414093046336No Hit
CCAAAAGCAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAA1135850.18233416399974425RNA PCR Primer, Index 1 (100% over 23bp)
GCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGCCA1066010.17112298469460524No Hit
CAGTGTACTCCGAAGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1042000.16726874049190782RNA PCR Primer, Index 1 (100% over 30bp)
AGCGTGTACTCCGAAGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT1007600.16174662468296194RNA PCR Primer, Index 1 (100% over 30bp)
AGCGTGTACTCCGAAGAGGATTGGAATTCTCGGGTGCCAAGGAACTCCAG992760.15936440960724224RNA PCR Primer, Index 1 (100% over 29bp)
TAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAAC938410.15063978768235242RNA PCR Primer, Index 1 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC908030.1457629888952659RNA PCR Primer, Index 1 (100% over 24bp)
AACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAGGAACTC868500.1394173715136487RNA PCR Primer, Index 1 (100% over 26bp)
TTAACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAGGAAC866080.13902889708755425RNA PCR Primer, Index 1 (100% over 24bp)
GGATCCAAAAGCAGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCA858350.13778802629676495No Hit
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA836840.13433509864995025RNA PCR Primer, Index 1 (100% over 28bp)
TTAACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAACTCC806790.12951127364824025RNA PCR Primer, Index 1 (100% over 27bp)
ATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGA804960.1292175099293341RNA PCR Primer, Index 1 (100% over 22bp)
AAAAGCAGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAAC788990.12665389977035543RNA PCR Primer, Index 1 (100% over 24bp)
AAAAGCAGTGTACTCCGAAGAGGTGGAATTCTCGGGTGCCAAGGAACTCC788170.1265222679400259RNA PCR Primer, Index 1 (100% over 27bp)
TTAACGCGAACTCGGCCTACAATATGGAATTCTCGGGTGCCAAGGAACTC771670.12387357867120011RNA PCR Primer, Index 1 (100% over 26bp)
AGGCATTAACGCGAACTCGGCCTACAATAGTGACTGGAATTCTCGGGTGC703940.11300111053922611No Hit
GCATTAACGCGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAG693880.11138621271835412No Hit
CGAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG687460.11035563180284737RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGTGTACTCCGAAGAGGACCTGGAATTCTCGGGTGCCAAGGAACTCC686540.11020794731028254RNA PCR Primer, Index 1 (100% over 27bp)
ACGCGAACTCGGCCTACAATAGTTGGAATTCTCGGGTGCCAAGGAACTCC667660.10717720468025642RNA PCR Primer, Index 1 (100% over 27bp)
AACGCGAACTCGGCCTACAATTGGAATTCTCGGGTGCCAAGGAACTCCAG662360.10632641358178509RNA PCR Primer, Index 1 (100% over 29bp)
GTTCAGAGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAAC660330.10600054453840835Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGCAGTGTACTCCGAAGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG655960.10529904319872539RNA PCR Primer, Index 1 (100% over 29bp)
GAACTCGGCCTACAATAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT651680.10461198925505423RNA PCR Primer, Index 1 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA647690.10397148803186543No Hit
GCCCGAGGTGGGATCCCGAGGCCTTGGAATTCTCGGGTGCCAAGGAACTC635080.10194724732399311RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph